LAMP assay for detection of Asian fish tapeworm

Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.

Categories: Data; Tags: Asian fish tapeworm, LAMP assay, USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... eDNA, environmental DNA, invasive species, isothermal amplification, loop-mediated isothermal amplification, molecular detection, parasites, rapid assay, Fewer tags

Data release for validation of a portable eDNA detection kit for invasive carps

This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33 Grass Carp (Ctenopharyngodon idella) mixed with thousands of baitfish. The standards and controls data collected with the eDNA sample analysis are split into separate files for each method to simplify the statistical analysis of the eDNA sample data.

Categories: Data; Tags: Genetics, USGS Science Data Catalog (SDC), biota, environmental DNA, grass carp, All tags... invasive carp, loop-mediated isothermal amplification, Fewer tags