Filters: Tags: methods development (X)
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Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder.
Categories: Data;
Tags: CONUS,
Emerging Infectious Disease,
Highly Pathogenic Avian Influenza Virus,
Methods Comparison,
Methods Development,
Poor survival, growth and reproduction of the amphipod Hyalella azteca has been observed in exposures using reconstituted waters. Previous studies have reported success in H. azteca water-only exposures with the addition of relatively high concentrations of bromide (Br). This study evaluated the influence of lower environmentally representative concentrations of Br on the response of the amphipod H. azteca in 42-d water-only exposure conditions in various reconstituted waters. Improved performance of H. azteca was observed in reconstituted waters with >0.02 mg Br/L. These tables and figures provide online supplemental information that will not be included in the journal article.
Categories: Data;
Types: Citation;
Tags: NA,
chronic testing,
methods development,
water-only testing
Studies have been conducted to refine USEPA, ASTM, and Environment Canada standard methods for conducting 42-d reproduction tests with H. azteca in water or in sediment. Modifications to the H. azteca method include better defined ionic composition requirements for exposure water (i.e., >15 mg/L of chloride and >0.02 mg/L of bromide) and improved survival, growth and reproduction with alternate diets provided as increased rations over time in water-only or whole-sediment toxicity tests. A total of 24 laboratories volunteered to participate in an inter-laboratory study evaluating the performance of H. azteca in 42-d studies in control sand or control sediment using the refined methods. Improved growth and reproduction...
The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among...
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