Filters: Tags: microbes (X) > partyWithName: Wesley A Bickford (X)
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To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
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