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These data sets present results from the analyses of groundwater samples collected from monitoring wells and monitoring intervals in bedrock wells in the mudstone aquifer underlying the former Naval Air Warfare Center (NAWC), West Trenton, NJ. The water samples were collected between 2008 and 2013 and were analyzed for field parameters, inorganic and organic constituents, and the abundances of selected microbes of importance to the evaluation of biological degradation of organic contaminants in groundwater. The collection and analyses of the groundwater samples coincides with conducting a bioaugmentation experiment in a targeted region of the mudstone aquifer. The purpose of the bioaugmentation was to introduce...
Our objective is to improve the scientific understanding of the modes, rates, and mechanisms of carbon stabilization and losses in soils from Alaska, California, and other Western states. We focus on the biophysical and microbial mechanisms that drive carbon gains and losses, and to use our data to improve models of soil carbon cycling. This catalog supports research from several projects focused on soil carbon cycling. It encompasses multiple types of datasets including environmental, ecological, biological, isotopic, mineralogical, genomic, flux, and modeled data from water, vegetation, soil, and atmospheric matrices. The catalog will be available online and to the public. Therefore, publication of data through...
OBIS-USA brings together marine biological occurrence data – recorded observations of identifiable marine species at a known time and place, collected primarily from U.S. Waters or with U.S. funding. Coordinated by the Science Analytics and Synthesis (SAS) Program of the United States Geological Survey (USGS), OBIS-USA, strives to meet national data integration and dissemination needs for marine data about organisms and ecosystems. OBIS-USA is part of an international data sharing network (Ocean Biodiversity Information System, OBIS) coordinated by the Intergovernmental Oceanographic Commission, of UNESCO (United Nations Educational, Science and Cultural Organization) International Oceanographic Data and Information...
Abstract The importance of snow and related cryospheric processes as an ecological factor has been recognized since at least the beginning of the twentieth century. Even today, however, many observations remain anecdotal. The research to date on cold-lands ecosystems results in scientists being unable to evaluate to what extent changes in the cryosphere will be characterized by abrupt changes in local and global biogeochemical cycles, and how these changes in seasonality may affect the rates and timing of key ecological processes. Studies of gas exchanges through snow have revealed that snow plays an important role in modulating wintertime soil biogeochemical processes, and that these can be the driving processes...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: Biogeochemistry,
Carbon dioxide,
Isotopes,
Microbes,
Nitrogen oxides,
Centaurea diffusa is one of the most destructive invasive weeds in the western USA and allelopathy appears to contribute to its invasiveness (Callaway & Aschehoug 2000). Here we identify a chemical from the root exudates of C. diffusa, 8-hydroxyquinoline, not previously reported as a natural product, and find that it varies biogeographically in its natural concentration and its effect as an allelochemical. 8-Hydroxyquinoline is at least three times more concentrated in C. diffusa-invaded North American soils than in this weed's native Eurasian soils and has stronger phytotoxic effects on grass species from North America than on grass species from Eurasia. Furthermore, experimental communities built from North American...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: 8-Hydroxyquinoline,
Centaurea diffusa,
Ecology Letters,
allelopathy,
biota,
Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but...
Categories: Data;
Tags: Barnstable County,
Cape Cod,
DNA sequencing,
Environmental Health,
Hydrology,
To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
Categories: Data;
Tags: Biochemistry,
DNA sequencing,
Energy Resources,
Environmental Health,
Fayette County,
The Permian Basin, straddling New Mexico and Texas, is one of the most productive oil and gas (OG) provinces in the United States. OG production yields large volumes of wastewater that contain elevated concentrations of major ions including salts (also referred to as brines), and trace organic and inorganic constituents. These OG wastewaters pose unknown environmental health risks, particularly in the case of accidental or intentional releases. Releases of OG wastewaters have resulted in water-quality and environmental health effects at sites in West Virginia (Akob, et al., 2016, Orem et al. 2017, Kassotis et al. 2016) and in the Williston Basin region in Montana and North Dakota (Cozzarelli et al. 2017, Cozzarelli...
While it is widely recognized that microorganisms are intimately linked with every biogeochemical cycle in all ecosystems, it is not clear how and when microbial dynamics constrain ecosystem processes. As a result, it is know clear how to apply the value of increasingly detailed characterization of microbial properties to our understanding of ecosystem ecology. Several recent papers have demonstrated how information about microbial dynamics can be incorporated into ecosystem models (Allison et al. 2010, McGuire and Treseder 2010, Todd - Brown et al. 2011a), but it is generally not clear what types of microbial data are most useful in explaining variation in biogeochemical processes and ecosystem functioning, especially...
We deployed a sampler to characterize water quality from a household well tapping a shallow fractured dolomite aquifer in northeast Wisconsin. The sampler was deployed from January to May 2017, and monitored temperature, nitrate, chloride, specific conductance, and fluorescent dissolved organic matter on a minute time step; water was directed to sequential microbial filters during three recharge periods that ranged from 5 to 20 days. Results from the automated sampler demonstrate the dynamic nature of the household water quality, especially with regard to microbial targets, which were shown to vary 1 to 2 orders of magnitude during a single sampling event. We believe assessments of pathogen occurrence and concentration,...
Categories: Data;
Tags: Door County,
Geochemistry,
USGS Science Data Catalog (SDC),
Wisconsin,
automated sampling,
OBIS-USA brings together marine biological occurrence data – recorded observations of identifiable marine species at a known time and place, collected primarily from U.S. Waters or with U.S. funding. Coordinated by the Core Science, Analytics, Synthesis, and Libraries (CSAS&L) Program of the United States Geological Survey (USGS), OBIS-USA, strives to meet national data integration and dissemination needs for marine data about organisms and ecosystems. OBIS-USA is part of an international data sharing network (Ocean Biogeographic Information System, OBIS) coordinated by the Intergovernmental Oceanographic Commission, of UNESCO (United Nations Educational, Science and Cultural Organization International Oceanographic...
Categories: Project;
Types: NSDI Cooperative Agreements Program Project;
Tags: Arctic Ocean,
Atlantic Ocean,
Bay of Fundy,
Beaufort Sea,
Bering Sea,
Plant roots have the best-understood mutualisms with microbes, but leaf and bract cell endosymbiosis have not been previously reported. Leaf and bract cells of more than 30 species in 18 families of seed plants were surveyed for the presence of intracellular bacteria and several experiments were designed to find and analyze nutrient exchanges between bacteria and plant cells. This dataset contains the results of 1) histochemical analyses to detect hormones, superoxide, and nitrogenous chemicals around bacteria within plant leaf and bract cells, 2) experiments to assess the differential absorption of isotopic nitrogen into plants, and 3) genetic analysis of bacteria isolated from plant material.
The data were gathered as a preliminary assessment of soil microbiology and conditions in selected urban stormwater best management practices (BMPs) in Clarksburg, MD. Four bioretention facilities (BF), four dry ponds (DP), and four surface sand filters (SSF) were selected. Three samples were taken from each BMP (a single sample from one dry swale (DS) was also collected). BMPs were selected based on their position along various stormwater treatment trains. Soil samples were taken after precipitation events in the summer of 2015 and analyzed for various soil chemistry parameters and microbial taxonomic profiling.
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Clarksburg,
Land Use Change,
Maryland,
Montgomery County,
Soil Sciences,
These datasets provide geographic sampling information for Bathymodiolin mussels collected from three cold seep sites off the mid-Atlantic U.S. coast, northwestern Atlantic Ocean. Also included are GenBank accession numbers for mitochondrial gene sequences (COI and ND4) and sequence alignments (File1_mussels) used in phylogenetic analyses for molecular identification of the mussel species. Additionally, GenBank accession numbers for the BioProject containing 16S metabarcoding sequence data used to identify gill symbionts in mussels are provided, along with supporting details for the analyses performed on the 16S metabarcoding data (File2_Symbionts).
A large spill of wastewater from oil and gas operations was discovered adjacent to Blacktail Creek near Williston, North Dakota in January 2015. To determine the effects of this spill on streambed microbial communities over time, bed sediment samples were taken from Blacktail Creek upstream, adjacent to, and at several locations downstream from the spill site. Blacktail Creek is a tributary of the Little Muddy River, and additional samples were taken upstream and downstream from the confluence of Blacktail Creek and the Little Muddy River. Samples were collected in February 2015, June 2015, June 2016, and June 2017. DNA was extracted from these sediments, and sequencing of the 16S ribosomal RNA gene was performed...
Categories: Data;
Tags: Aquatic Biology,
Blacktail Creek,
DNA sequencing,
Ecology,
Little Muddy River,
FY2014This project proposes to test the hypothesis that soil fungistasis (suppression of fungal pathogens by soil microbes in carbohydrate-limited soil) and its alleviation through natural carbohydrate augmentation (e.g., cheatgrass litter, leakage from cheatgrass roots) are the principal processes mediating patterns of cheatgrass die-off and recovery in die-off-prone areas.The project team will use laboratory, greenhouse, and field manipulative experiments to examine the effect of soil carbohydrates on cheatgrass disease incidence.
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