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To test the effect of geochemical heterogeneity on microorganism transport in saturated porous media, we measured the removal of two microorganisms, the bacteriophage PRD1 and oocysts of the protozoan parasite Cryptosporidium parvum, in flow-through columns of quartz sand coated by different amounts of a ferric oxyhydroxide. The experiments were conducted over ranges of ferric oxyhydroxide coating fraction of lambda = 0-0.12 for PRD1 and from lambda = 0-0.32 for the oocysts at pH 5.6-5.8 and 10(-4) M ionic strength. To determine the effect of pH on the transport of the oocysts, experiments were also conducted over a pH range of 5.7-10.0 at a coating fraction of lambda = 0.04. Collision (attachment) efficiencies...
Categories: Publication;
Types: Citation;
Tags: Animals,
Bacteriophage PRD1,
Biological Transport,
Cryptosporidium parvum,
Electron,
Although cyclosporine has high specificity for the immune system, immunosuppressive therapy with CsA is often complicated by nephrotoxicity. The main morphologic targets of CsA nephrotoxicity include the tubular epithelial and endothelial cells. These cells were investigated in vitro. CsA caused a dose- and time-dependent inhibition of cell growth, vacuolization and fatty change in adherent cells, detachment, and cell death. Inhibition of 3H-TdR incorporation in cells of both tubular epithelial and endothelial origin occurred between 3 microM and 10 microM. Electron microscopy studies revealed cellular swelling, dilatation of the endoplasmic reticulum, and the presence of lipid droplets and giant mitochondria. The...
Categories: Publication;
Types: Citation;
Tags: Animals,
Carrier Proteins,
Cell Division,
Cell Line,
Cyclosporins,
Here we compare pollen identification results derived from light microscopy and DNA sequencing techniques of a robust number of samples collected from honey bee colonies embedded within intensive agricultural landscapes in the Northern Great Plains. We collected pollen samples from colonies within 6 apiaries in 2010 and 2011. For each pollen sample, we identified pollen grains via light microscopy and provide the number of grain counts-per-million. A separate aliquot of each pollen sample subjected to light microscope identification was also used for DNA sequencing analysis. We provide the plant operational taxonomic unit (OTU) for all base pair reads as the number of reads-per-million.
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