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Tree swallow (Tachycineta bicolor) eggs and nestlings were collected from 16 sites across the Great Lakes to quantify normal annual variation in total polychlorinated biphenyl (PCBs) exposure and to validate the sample size choice in earlier work. A sample size of 5 eggs or 5 nestlings per site was adequate to quantify exposure to PCBs in tree swallows given the current exposure levels and variation. There was no difference in PCB exposure in 2 randomly selected sets of 5 eggs collected in the same year, but analyzed in different years. Additionally, there was only modest annual variation in exposure, with between 69% (nestlings) and 73% (eggs) of sites having no differences between years. There was a tendency,...
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Analysis of biological tissues or direct sources of food may better characterize exposure of non-target organisms to current-use pesticides. Food boluses were collected from tree swallow (Tachycineta bicolor) nestlings using a non-lethal, ligature method and were composited based on nest. The ligatures, which prevented the bolus from being swallowed, were placed on nestlings for 1 h until samples were collected. Samples in 2016 were collected from nestlings at 6 days and 12 days post-hatch whereas 2018 samples were collected from nestlings at 12 days post-hatch. Furthermore, in 2016 and 2018, insects commonly consumed by tree swallows were collected via sweep net and composited based on suborder (Brachycera, Nematocera,...


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