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The nitrogen-fixing lichen Pettigera aphthosa (L.) Willd. was treated under semi-natural conditions with simulated rain containing different combinations of ammonium, nitrate and sulphuric acid. Nitrogen in neutral solution had no negative effect on the nitrogen fixation rate. Sulphuric acid had a negative effect on nitrogen fixation rate, especially in combination with ammonium. The results could suggest an explanation for the sudden decline of P. aphthosa in southern Sweden. Published in New Phytologist, volume 120, issue 1, on pages 99 - 103, in 1992.