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Abstract: Freshwater mussels are among the most imperiled faunal groups globally, and relevant ecological information is urgently needed to guide their management and conservation in the face of global change. We explored the influence of species traits, host fishes, and habitat at three spatial scales (micro-, reach-, and catchment-scale) on the detection and occupancy of 14 species of freshwater mussels in the Tar River basin, North Carolina. Detection probability for all species was 0.42 (95% CI, 0.36 –0.47) with no species- or site-specific detection effects identified. Mean occupancy probability among species ranged from 0.04 (95% CI, 0.01 – 0.16) for Alasmidonta undulata, an undescribed Lampsilis sp., and...