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Stable isotope dynamics of herbivorous reef fishes and their ectoparasites: 2012, 2013, 2018 Acanthurid surgeonfishes are an abundant and diverse group of herbivorous fishes on coral reefs. While their contribution to trophic linkages and dynamics in coral reef systems has received considerable attention, the role of linkages involving their parasites has not. As both consumers of fish tissue and prey to microcarnivores, external parasites can play a significant role in trophic transfer between the primarily algae-based food source and the broader coral reef community. Stable isotope analysis is a common tool for studying trophic linkages, which can be used for studies involving parasites. These data were collected to examine the stable isotope ecology (13C and 15N) of copepod (Caligus atromaculatus) and... Categories: Data; Tags: Aquatic Biology, Ecology, Ecology, USGS Science Data Catalog (SDC), Virgin Islands of the United States, All tags... Wildlife Biology, Wildlife Biology, Wildlife Disease, Wildlife Disease, coral reefs, farming, herbivores, oceans, parasites, Fewer tags Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2... Categories: Data; Tags: Hawaii, USGS Science Data Catalog (SDC), Wildlife Disease, climatologyMeteorologyAtmosphere, parasites, All tags... pathology, spirorchiid, Fewer tags Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA. Categories: Data; Tags: Montana, Upper Yellowstone, aquatic biology, biota, parasites Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019 This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA. Categories: Data; Tags: California, Idaho, Montana, Upper Snake, Upper Yellowstone, All tags... aquatic biology, farming, parasites, Fewer tags Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2020 There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019 and 74 sites in spring 2020. Our surveillance identified zones with high waterborne parasite loads and provides a proof of concept for this approach that could be expanded throughout the larger Lake Washington watershed. Categories: Data; Tags: Aquatic Biology, Ceratonova shasta, Ecology, Parvicapsula minibicornis, Puget Sound, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Washington, Wildlife Disease, biota, environment, fishes, inlandWaters, parasites, Fewer tags Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA. Categories: Data; Tags: Montana, Upper Yellowstone, aquatic biology, biota, parasites Carcass weights, 28S rRNA alignment file and parasite sample vouchers collected from California tiger salamanders (Ambystoma californiense) CTS and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) SCLT from Prospect or Ellicott Pond, on Ellicott Slough National Wildlife Refuge, California U.S.A. recorded September 11, 2019 Animal identification number, weight in grams for carcasses of California tiger salamanders (Ambystoma californiense) CTS and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) SCLT from Prospect or Ellicott Pond, on Ellicott Slough National Wildlife Refuge, California U.S.A. submitted to U.S. Geological Survey, National Wildlife Health Center (NWHC) for determination of cause of death. NWHC Case 29381. MSF Sequence file used to construct a phylogenetic tree. Sequences were generated from samples at NWHC (Px) and using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) they were compared for base pair similarities with publicly available sequences from GenBank. Sequences selected for the Multi Sequence... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: California tiger salamanders, Ellicott Slough National Wildlife Refuge, Ribeiroia ondatrae, Santa Cruz long-toed salamander, U.S. Geological Survey, National Wildlife Health Center, Madison, Wisconsin, All tags... USGS Science Data Catalog (SDC), Wildlife Disease, biota, parasites, Fewer tags DNA sequences from Ichthyophonid parasites Data included are DNA sequences used to identify species boundaries among Ichthyophonus-like parasites. Sequences were generated for three gene regions from each parasite isolate. DNA sequences can be downloaded from GenBank (https://www.ncbi.nlm.nih.gov/) as text or fasta files. Accession numbers for each sequence are included in the data table (.csv file). Data includes 87 sequences (99,383 total nucleotides). Once manuscript is accepted there are no restrictions on dissemination of these data. Categories: Data; Tags: Aquatic Biology, Bering Sea, DNA sequencing, Environmental Health, Genetics, All tags... North Atlantic, Northeast Pacific, Ocean, Puget Sound, USGS Science Data Catalog (SDC), USGS-EMA-LOW Fish and Wildlife Disease, Wildlife Disease, Yukon, farming, parasites, wildlife disease, Fewer tags Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. Categories: Data; Types: Citation; Tags: California, Idaho, Montana, PCR, Proliferative Kidney Disease, All tags... Tetracapsuloides bryosalmonae, Wyoming, environmental DNA, fish, molecular biology, parasites, Fewer tags Host feeding ecology and trophic position significantly influence isotopic discrimination between a generalist ectoparasite and its hosts: implications for parasite-host trophic studies Despite being one of the most prevalent forms of consumerism in ecological communities, parasitism has largely been excluded from food-web models. Stable isotope analysis of consumers and their diets has been widely used in the study of food-webs for decades. However, the amount of information regarding parasite stable isotope ecology is limited, restricting the ability of ecologists to use stable isotope analysis to study parasites in food-webs. This study took advantage of distinct differences in the feeding ecology and trophic position of different species of fish known to host the same common micropredatory gnathiid isopod, to study the effects of host stable isotope ecology on that of the associated micropredator.... Categories: Data; Tags: Eastern Caribbean, Ecology, Shallow/Mesophotic Coral Reef Biota, St John, United States Virgin Islands, Stable Isotopes, All tags... USGS Science Data Catalog (SDC), Virgin Islands of the United States, biota, coral reefs, food web, parasites, stable isotope analysis, symbiosis, Fewer tags Pathogenic lineage of Perkinsea causes mass mortality of frogs across the USA: Data (i) Severe perkinsea data was based on the observation of characteristic gross and microscopic lesions and the morphological identification of Perkinsea organisms within the lesions. Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (ii) Epidemiological information was compiled from each anuran sample, including the detection type (mortality event or health monitoring study), location (state and county), anuran species reported in the event, life-stage of species reported in the... Categories: Data; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: USGS Science Data Catalog (SDC), United States, amphibians, disease vectors, health and disease, All tags... parasites, wildlife mortality, Fewer tags LAMP assay for detection of Asian fish tapeworm Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls. Categories: Data; Tags: Asian fish tapeworm, LAMP assay, USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... eDNA, environmental DNA, invasive species, isothermal amplification, loop-mediated isothermal amplification, molecular detection, parasites, rapid assay, Fewer tags Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification. Categories: Data; Types: Citation; Tags: East Gallatin River, Montana, Sypes Canyon Creek, Tetracapsuloides bryosalmonae, Yellowstone River, All tags... biota, environmental DNA, molecular biology, myxozoan, parasites, qPCR, Fewer tags Environmental DNA robotic and manual sampling data, Yellowstone and Snake Rivers, 2017-2019 Environmental DNA detection results from samples collected using autonomous water sampling robots and manual approaches. Samples were collected in the Upper Yellowstone River (Montana) and Upper Snake River (Idaho/Wyoming) in 2018 and 2019. Samples were tested for the DNA of the following species: the waterborne protozoa Naegleria spp., the fish pathogen Tetracapsuloides bryosalmonae, Scomber japonicas (mackerel fish), kokanee salmon (Oncorhynchus nerka) and dreissenid mussel (Dreissena spp.). Categories: Data; Tags: Palisades Dam, USGS Science Data Catalog (SDC), Upper Snake, Upper Yellowstone, Yellowstone National Park North, All tags... biota, environmental DNA, invasive species, parasites, river systems, Fewer tags Parasite sample voucher numbers and 28S rRNA alignment file for parasites collected from California Giant Salamander (Dicamptodon ensatus) from Webb Creek, in the Bear Creek Redwoods Preserve in Santa Clara County, California, USA Multi sequence file (.msf) file used to construct a 28S rRNA phylogenetic tree of species of Euryhelmis and other heterophyid trematodes. Sequences were generated from metacercariae samples at U.S. Geological Survey National Wildlife Health Center (NWHC) and compared for base pair similarities with publicly available sequences from GenBank using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Selection of GenBank sequences to include in the .msf file was based on the number of base pair similarities (94% or higher identity to this study’s sequences), 98–100% coverage AND stage of parasite from which the sequence was generated. An excel file listing all parasites and DNA from NWHC vouchered with the University... Categories: Data; Tags: Bear Creek Redwoods Preserve, California Giant Salamander, Euryhelmis sp, Santa Clara, USGS Science Data Catalog (SDC), All tags... Webb Creek, Wildlife Disease, biota, infection, parasites, Fewer tags Caligus Clemensi prevalence and counts on Clupea pallasii from Port Angeles Harbor, WA and from a controlled laboratory experiment conducted at USGS Marrowstone Marine Field Station, WA We characterized a natural sea louse epizootic of Caligus clemensi and the effects of parasitism on Pacific herring Clupea pallasii in Port Angeles Harbor, WA, USA. Infestation prevalence on newly metamorphosed age0 Pacific herring reached 100% prevalence by mid-August. At this time, the mean louse intensity was 4.6 lice and a positive correlation occurred between louse intensity and Pacific herring body length. The epizootic then waned, with infestation prevalence decreasing to less than 25% and the mean parasite intensity falling below 1 louse by October. In controlled experiments, louse-induced mortality was negligible in controlled experiments. While skin injuries were not detected, motile lice preferentially... Categories: Data; Tags: Pacific Northwest, Port Angeles, USGS Science Data Catalog (SDC), biota, fish, All tags... health and disease, parasites, Fewer tags