Filters: Tags: parasites (X) > Types: Citation (X)
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Categories: Data;
Types: Citation;
Tags: California,
Idaho,
Montana,
PCR,
Proliferative Kidney Disease,
(i) Severe perkinsea data was based on the observation of characteristic gross and microscopic lesions and the morphological identification of Perkinsea organisms within the lesions. Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (ii) Epidemiological information was compiled from each anuran sample, including the detection type (mortality event or health monitoring study), location (state and county), anuran species reported in the event, life-stage of species reported in the...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: USGS Science Data Catalog (SDC),
United States,
amphibians,
disease vectors,
health and disease,
A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.
Categories: Data;
Types: Citation;
Tags: East Gallatin River,
Montana,
Sypes Canyon Creek,
Tetracapsuloides bryosalmonae,
Yellowstone River,
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