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Viral hemorrhagic septicemia virus (VHSV) is an aquatic rhabdovirus causing severe disease in numerous freshwater and saltwater fish species. Initially the virus was been found to cause disease in European fish populations starting around 1938 and was first detected in North America in the late 1980s. Of the four VHSV genotypes (I, II, III, and IV), the North American subtype IVb isolates have a broad host range. To determine whether endangered pallid sturgeon Scaphirhynchus albus, are susceptible to VHSV-IVb infection, juvenile pallid sturgeon and two pallid sturgeon cell lines derived from skin and spleen tissue were tested. Detection of viable virus via a plaque assay and molecular detection of the virus results...