Pseudogymnoascus destructans survival at elevated temperatures – Artificial media count data

The survival of Pseudogymnoascus destructans (Pd) was evaluated at temperatures outside of its thermal range of growth on three different artificial growth media; Sabouraud dextrose agar (SD), brain-heart infusion agar (BHI), and brain-heart infusion agar supplemented with 10% sheep red blood cells (BHI+B). Pd was harvested from starting cultures grown of MEA agar at 7˚C for 60 days. Harvested conidia were diluted in Phosphate Buffered Saline + Tween20 and spread onto plates of a given medium. Plate were then incubated at either 24, 30 or 37˚C. Plates were incubated for 1, 5, 9, 15, 30, 60, 90, 120, or 150 days before being transferred to a 7˚C incubator for 50 days. Colony forming units (CFUs) of Pd were then enumerated,...

Categories: Data; Tags: Fungal pathogens, Pseudogymnoascus destructans, U.S. Geological Survey National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, pathogen survival, persistence, temperature, white-nose syndrome, Fewer tags