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Pollen was measured in ambient air by several methods and in wet atmospheric deposition samples at three monitoring sites in the National Atmospheric Deposition Program (NADP) National Trends Network. A method for counting pollen on filters was developed and provided pollen counts for NADP atmospheric wet-deposition samples and high-volume ambient air samplers (HVAS) for comparison with co-located traditional microscopy and PollenSense sensor counting methods during the 2021 pollen season. Air and precipitation samples were collected by the NADP and analyzed by the Wisconsin State Laboratory of Hygiene, in Madison, Wisconsin and Aerobiology Research Laboratories (Canada). Daily data were obtained from online summaries...
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Pollen morphology and pollen morphology in conjunction with other characteristics have elucidated the origin and evolution of various plant groups. Many studies of fossil pollen rarely discuss the effects of diagenesis and sample preparation on pollen characteristics. This paper examines the relationship of diagenesis, sample preparation (e.g., acetolysis), and staining techniques on the preservation and interpretation of pollen characteristics viewed with light, scanning and transmission electron microscopy. Based on empirical studies, different species under similar experimental conditions have different reflectance values, indicating the individual species respond differently to diagenetic events. The quantitative...
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In this study US Geological Survey scientists collected pollen samples from honey bee (Apis mellifera) colonies distributed across North Dakota, South Dakota, and Minnesota, in 2015 and 2016. Sequencing of nuclear ribosomal loci was used to determine the number and abundance of flowering plant taxa. The data contain counts of Operational Taxonomic Unit (OTU) reads of honey bee collected pollen from apiary locations throughout the growing season.
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Here we compare pollen identification results derived from light microscopy and DNA sequencing techniques of a robust number of samples collected from honey bee colonies embedded within intensive agricultural landscapes in the Northern Great Plains. We collected pollen samples from colonies within 6 apiaries in 2010 and 2011. For each pollen sample, we identified pollen grains via light microscopy and provide the number of grain counts-per-million. A separate aliquot of each pollen sample subjected to light microscope identification was also used for DNA sequencing analysis. We provide the plant operational taxonomic unit (OTU) for all base pair reads as the number of reads-per-million.


    map background search result map search result map A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding datasets Dataset: Molecular identification of honey bee collected pollen in the Northern Great Plains, North America, 2015-2016 Reflectance and spectral fluorescence data from the effect of diagenesis on the preservation of morphology and ultrastructural features of pollen National Atmospheric Deposition Program Pollen Study Data for 2021 Pollen Season A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding datasets Dataset: Molecular identification of honey bee collected pollen in the Northern Great Plains, North America, 2015-2016 National Atmospheric Deposition Program Pollen Study Data for 2021 Pollen Season Reflectance and spectral fluorescence data from the effect of diagenesis on the preservation of morphology and ultrastructural features of pollen