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This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
Fragment data from 11 nuclear microsatellite loci and sequence data from the mitochondrial cytochrome b gene were gathered from Arctic cod (Boreogadus saida) in the Beaufort and Chukchi seas. Mitogenomic analyses of Arctic cod and three other co-distributed gadoids (Polar cod [Arctogadus glacialis], Saffron cod [Eleginus gracilis], Walleye Pollock [Gadus chalcogrammus]) were also completed. Transcriptomic analyses of Arctic cod were used to identify genes associated with environmental perturbations. Genome-wide scans using RAD-Seq of Arctic cod and the co-distributed Polar Cod were collected.
Categories: Data;
Tags: Alaska,
Aleutian Islands,
Aquatic Biology,
Aquatic ecosystems,
Aquatic sciences,
This data set describes nuclear microsatellite genotypes derived from six autosomal loci (AgCA222, Age7.1JT, BV13, BV20, NVH206, NVH195-2) in Cooper's Hawks. Samples originated from blood samples collected from live trapped birds. Version History: First release: December 2020 Revised: February 2022 (ver. 2.0)
Categories: Data,
Data Release - Revised;
Tags: Accipiter cooperii,
AgCA222,
Age7.1JT,
Alleles,
Animals/Vertebrates,
The dataset contains Esherichia coli and quantitative polymerase chain reaction data for microbial source tracking markers in the Chattahoochee River and streams within the Chattahoochee River National Recreation area from samples collected in the winter and summer of the federal fiscal years 2016 and 2017. Six water samples were collected from three sites on the Chattahoochee River each season. Eleven stream sites and one additional site on the Chattahoochee River were sampled once each season, with the exception of two sites, which were not sampled in winter FY16.
Categories: Data;
Tags: Atlanta,
Chattahoochee,
Environmental Health,
USGS Science Data Catalog (SDC),
Water Resources,
These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.
Categories: Data;
Tags: Alaska,
Amino Acid Oxidoreductases,
Animals/Invertebrates,
Biodiversity,
Biodiversity,
This data set contains sampling information, allele sizes of 11 microsatellite loci, and Genbank accession numbers of ddRAD seq results for scoters (Melanitta sp.). Microsatellite data is provided for three North American species of scoter (black scoter, M. americana n = 61; white-winged scoter, M. deglandi, n = 208; surf scoter, M. perspicillata, n = 145) and their European congeners (common scoter, M. nigra, n = 19; velvet scoter, M. fusca, n = 20). Individuals with ddRAD results include 27 black scoters, 4 common scoters, 32 surf scoters, 28 white-winged scoters, and 4 velvet scoters.
Avian botulism toxicity is a common cause of death to water and shore birds that live near or migrate through Lake Michigan. The botulism neuro-toxin type E (bontE) gene is responsible for the production of botulinum neurotoxin type E. Quantitative Polymerase Chain Reaction (qPCR) was performed using a Step One Plus Thermocycler (Applied Biosystems) and protocol described in Getchell and others, 2011, Journal of Aquatic Animal Health. The assay was used to assess microbial community DNA obtained from environmental samples that were collected by Great Lakes Science Center and by National Park Service from 2011 to 2014 for the bontE gene. Samples were obtained by ponar grab or by divers and matrices collected...
This data set contains genetic information collected from eelgrass (Zostera marina) populations along the Pacific coast of North America from Alaska to Baha California. A total of 447 samples were collected comprising 401 unique individuals (genets) and 46 clones (ramets) from which 10 microsatellite DNA loci were obtained.
Categories: Data;
Types: Citation;
Tags: Alleles,
Angiosperms (Flowering Plants),
Aquatic Biology,
Aquatic ecosystems,
Bahia Magdelena,
These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx...
Categories: Data;
Tags: USGS Science Data Catalog (SDC),
Wildlife Disease,
biota,
health,
microbiology,
Water samples from the Platte River were collected and analyzed for environmental DNA (eDNA). The U.S. Geological Survey (USGS) Nebraska Water Science Center (NEWSC) collected samples monthly from November 2019 to September 2022 at the Platte River near Ashland, Nebraska (USGS: 06801000). The USGS, in cooperation with the City of Lincoln, collected these samples. Water from the Platte River was collected as a series of weighted bottle subsamples that were taken from equal width increments across the river and composited into a sterilized 1-liter bottle. An aliquot of this composited sample was extracted using a sterile syringe and then filtered by the USGS NEWSC staff using filters provided by Jonah Ventures. These...
This data set provides sample information, microsatellite genotype, and NCBI accession numbers for mitochondrial DNA sequences of peregrine falcons (Falco peregrinus) from North America and Russia. Version History: First release: January 2020 Revised: June 2022 (ver. 2.0)
Categories: Data,
Data Release - Revised;
Tags: Alaska,
Alberta,
Alleles,
Animals/Vertebrates,
Biodiversity,
This dataset provides cycle threshold (Ct) values achieved during qPCR for 6 sex-linked genes, and two autosomal genes, for individuals from 60 species and across 13 orders of birds.
This dataset contains sampling information and genetic data of 12 novel microsatellite loci developed and polymorphic in the Sea Sandwort (Honckenya peploides) and screened in Merckia (Wilhelmsia physodes) from Alaska. The allele sizes for the 12 microsatellite loci developed in this study are included for two populations of Sea Sandwort (Fossil Beach, Kodiak and Egg Bay, Atka) and one population of Merckia (upper Coleen River, Brooks Range).
Categories: Data;
Tags: Alaska,
Aleutian Islands,
Angiosperms (Flowering Plants),
Biogeography,
Biota,
These data are comprised of two tables, one table containing wolverine (Gulo gulo) sample and genetic information for 20 microsatellite loci (Gg10-1, Gg25, Gg37-2, Gg42-1, Gg192-1, Gg443, Gg452, Gg454, Gg465, Gg471, Gg473, Gg-3-1, Gg-4, Gg-7-1, Ggu_216-1, Lut604, Ma-3-1, Mvis075, Tt-1, and Tt-4) and genetic sex determination results from North America and one Russian wolverine. All samples were obtained from collections at the University of Alaska Museum of the North or the University of New Mexico’s Museum of Southwestern Biology. The second table provides complete primer information for the genetic markers since several were redesigned from their originally-published sequences for this study.
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic...
Categories: Data;
Tags: Chlorodrepanis virens,
ELISA,
Hakalau Forest National Wildlife Refuge,
Hawaii,
Hawaii Amakihi,
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
This data set describes nuclear microsatellite genotypes derived from eight autosomal loci (Aph02, Aph11, Aalµ1, Sfiµ4, Smo4, Smo7, Smo12, and Sfiµ8) and accession numbers for double digest restriction-site associated DNA (ddRAD) sequences.
This data package is comprised of three tables with data from wintering Harlequin Ducks (Histrionicus histrionicus) from Alaska, British Columbia, Washington, Maine, Greenland, and Iceland. The first table contains available allele sizes for 7 microsatellite loci (Sfiµ1, Sfiµ4, Sfiµ9, Sfiµ10, Bcaµ10, Hhiµ2, Hhiµ5) and mtDNA control region haplotypes. The second table contains the mtDNA control region sequences used to assign haplotypes. The third table provides the geographic locations of sampling sites.
These are genetic data collected from over 700 individual coho salmon (Oncorhynchus kisutch) from 17 streams and rivers within Glacier Bay Alaska and 2 rivers outside the bay. Data collected from all samples include one nuclear gene intron, Growth Hormone-1, and eight microsatellite loci.
Categories: Data;
Tags: Alaska,
Animals/Vertebrates,
Aquatic Biology,
Aquatic ecosystems,
Aquatic sciences,
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm.
Categories: Data;
Tags: Chlorodrepanis virens,
ELISA,
Hakalau Forest National Wildlife Refuge,
Hawaii,
Hawaii Amakihi,
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