Research Microbiologist
Email:
catkinson@usgs.gov
Office Phone:
808-985-6401
Fax:
808-967-8568
ORCID:
0000-0002-4232-5335
Location
Bldg 344 Chain Of Craters Rd.
Hawaii National Park
, HI
96718
US
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We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured...
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This data release includes metadata and tabular data that documents initial water conditions (temperature, pH, dissolved oxygen and salinity) during aquarium trials testing the efficacy of carbon dioxide (CO2) to manage invasive fish in anchialine pools.
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This data release documents specificity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
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Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps...
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Management, All tags...
Metrosideros polymorpha,
Ohia lehua,
ROD,
Rapid Ohia Death,
Spore Traps,
environment, Fewer tags
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This data release includes metadata and tabular data that documents the survival of fish or invertebrate exposed to carbon dioxide (CO2) gas treatment during aquarium trials. Aquarium trials were conducted with six taxa: guppies (Poecilia reticulata), western mosquitofish (Gambusia affinis), Mozambique tilapia (Oreochromis mossambicus), Opae ula shrimp (Halocaridina rubra), feeble shrimp (Palaemon debilis) and a species of thiarid snail (Thiaridae). These results were part of a larger study aimed to test the efficacy of CO2 as a tool for managing invasive fish in anchialine pools.
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