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Characterizing the distribution of an endangered salmonid using environmental DNA analysis Determining species distributions accurately is crucial to developing conservation and management strategies for imperiled species, but a challenging task for small populations. We evaluated the efficacy of environmental DNA (eDNA) analysis for improving detection and thus potentially refining the known distribution of Chinook salmon (Oncorhynchus tshawytscha) in the Methow and Okanogan Subbasins of the Upper Columbia River, which span the border between Washington, USA and British Columbia, Canada. We developed an assay to target a 90 base pair sequence of Chinook DNA and used quantitative polymerase chain reaction (qPCR) to quantify the amount of Chinook eDNA in triplicate 1-L water samples collected at 48 stream... Categories: Data, Publication; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: EARTH SCIENCE > LAND SURFACE > LANDSCAPE, Federal resource managers, LCC Network Science Catalog, Publication, State agencies, All tags... Tribes, completed, environment, Fewer tags