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Water samples were collected from six cave locations where Actinobacterial mats appeared to be plentiful. Community-level physiological capabilities were evaluated using Biolog-Ecolog plates inoculated with cave water dosed with 0 or 0.10 milligrams per liter (mg/L) of erythromycin. The data were transformed into average well color development (AWCD). The transformation is done by subtracting the background color development (blank controls) and then averaging the three color readings of the same treatment on the same Biolog EcoPlate. Each EcoPlate has three replicates of 31 different substrates (potential bacterial food) that the microbial community can potentially metabolize and thereby transform a clear dye into...
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The U.S. Geological Survey, in cooperation with the Tennessee Department of Transportation, conducted an investigation of acid-rock drainage from road cuts in Tennessee during 2014-2015. The Devonian Chattanooga Shale contains disseminated pyrite and is a primary producer of acid-rock drainage (ARD) in Tennessee. One objective of the overall investigation was to attenuate ARD by manipulating the indigenous microbial community through different treatment injections. The scope of the study included establishing flow-through microcosms constructed with shale from the Chattanooga Shale formation rich in pyrite collected from an ARD site in Middle Tennessee. The microcosms were subjected to various treatments and evaluations...


    map background search result map search result map Average well color development data for water samples from six locations within the historic section of Mammoth Cave National Park, Kentucky Biogeochemical data from microcosm experiments to evaluate treatment of acid-rock drainage in selected counties in Tennessee Average well color development data for water samples from six locations within the historic section of Mammoth Cave National Park, Kentucky Biogeochemical data from microcosm experiments to evaluate treatment of acid-rock drainage in selected counties in Tennessee