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Filters Date Range Past year (1) Past month (0) Past week (0) Past day (0) Custom range... To   Search Contacts National Wildlife Health Center Erik K Hofmeister Susan Knowles U.S. Geological Survey - ScienceBase Ecosystems More... Categories Data (3) Tag Types Theme (3) Place (3) Harvest Set (3) USGS Scientific Topic Keyword (3) Tag Schemes None (3) USGS Thesaurus (2) ISO 19115 Topic Category (2) Common geographic areas (1) Marine Realms Information Bank (MRIB) keywords (1) View Results as: JSON ATOM CSV

A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Clinch River, USA. Histopathology and transmission electron microscopy showed a novel microsporidian parasite primarily infecting the ovary of pheasantshell. Sequencing of the small subunit rRNA gene produced a 1333 bp sequence with greatest similarity to Pseudonosema cristatellae (AF484694.1; 86.36%; e-value = 0), a microsporidium... Categories: Data; Tags: Actinonaias pectorosa, Clinch River, Virginia and Tennessee, USA, Microsporidium clinchi n. sp., PCR, TEM, All tags... USGS Science Data Catalog (SDC), Wildlife Disease, biota, bivalves, freshwater mussel, histopathology, microsporidia, unionid, Fewer tags LAMP assay for detection of Asian fish tapeworm Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls. Categories: Data; Tags: Asian fish tapeworm, LAMP assay, USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... eDNA, environmental DNA, invasive species, isothermal amplification, loop-mediated isothermal amplification, molecular detection, parasites, rapid assay, Fewer tags Parasite sample voucher numbers and 28S rRNA alignment file for parasites collected from California Giant Salamander (Dicamptodon ensatus) from Webb Creek, in the Bear Creek Redwoods Preserve in Santa Clara County, California, USA Multi sequence file (.msf) file used to construct a 28S rRNA phylogenetic tree of species of Euryhelmis and other heterophyid trematodes. Sequences were generated from metacercariae samples at U.S. Geological Survey National Wildlife Health Center (NWHC) and compared for base pair similarities with publicly available sequences from GenBank using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Selection of GenBank sequences to include in the .msf file was based on the number of base pair similarities (94% or higher identity to this study’s sequences), 98–100% coverage AND stage of parasite from which the sequence was generated. An excel file listing all parasites and DNA from NWHC vouchered with the University... Categories: Data; Tags: Bear Creek Redwoods Preserve, California Giant Salamander, Euryhelmis sp, Santa Clara, USGS Science Data Catalog (SDC), All tags... Webb Creek, Wildlife Disease, biota, infection, parasites, Fewer tags