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Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder.
We reviewed pathological findings and to a lesser extent epidemiological data from 70 free-ranging columbiforms naturally infected with Pigeon paramyxovirus-1 (PPMV-1) from 25 different PPMV-1 mortality events in columbiforms in the USA. In a subset of 17 birds from 10 of the studied outbreaks, we carried out immunohistochemistry targeting PPMV-1 nucleoprotein to determine the tissue distribution of the virus.
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Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR surveys of other Hawaiian geese with various pathologies, avian pox cases, and pox viral isolates failed to reveal REV suggesting the virus is uncommon, at least in samples examined. The full genome of the Gag, Pol, and Env genes were sequenced for all three infected birds and revealed geographic divergence of the Pol gene suggesting...
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We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and cleared the virus before the end of the study with no obvious clinical signs of disease. We additionally found no evidence of viral transmission to uninoculated subjects. These results indicate that while TABR are susceptible to SARS-CoV-2 infection, infection of wild populations of TABR would not likely cause mortality.
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This data set provides biological information from of a captive study of transgenic mice that received either passive serum transfers from black-footed ferrets (BFFs) that were vaccinated against SARS-CoV-2 or BFFs that were unvaccinated, or a monoclonal antibody against SARS-CoV-2 or lastly a placebo. After these treatments mice were challenged with SARS-CoV-2. The data included here have viral loads in the lungs of treated mice, as well as histological results from various tissues collected at the end of the study.
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In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, raccoons, and skunks, 261 (72%) of the animals harbored at least one coronavirus at the time. Among the samples which could be further characterized, 126 alphacoronaviruses and 88 betacoronaviruses (including 74 detections of SARS-CoV-2) were identified. Moreover, at least 10% (n=27) of the corona-virus-positive animals were found to be co-infected with more than one coronavirus....
Rabbit hemorrhagic disease, a notifiable foreign animal disease in the USA, was reported for the first time in wild native North American rabbits and hares in April 2020 in the southwestern USA. Affected species included the Desert Cottontail (Sylvilagus audubonii), Mountain Cottontail (S. nutallii), Black-tailed Jackrabbit (Lepus californicus), and Antelope Jackrabbit (L. alleni). Desert Cottontails (n=7) and Black-tailed Jackrabbits (n=7) collected in April and May 2020 were necropsied at the U.S. Geological Survey National Wildlife Health Center (NWHC) and tested positive for Lagovirus europaeus GI.2, also known as rabbit hemorrhagic disease virus 2 (GI.2/RHDV2/b), by real-time PCR at the U.S. Department of Agriculture’s...


    map background search result map search result map Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America” Data set on reticuloendotheliosis in Hawaiian birds Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America” Data set on reticuloendotheliosis in Hawaiian birds Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs