Filters: partyWithName: U.S. Geological Survey (X) > partyWithName: Maureen Purcell (X)
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There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019 and 74 sites in spring 2020. Our surveillance identified zones with high waterborne parasite loads and provides a proof of concept for this approach that could be expanded throughout the larger Lake Washington watershed.
Categories: Data;
Tags: Aquatic Biology,
Ceratonova shasta,
Ecology,
Parvicapsula minibicornis,
Puget Sound,
Piscine orthoreovirus genotype one (PRV-1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon, and rainbow trout injected with tissue homogenates from Atlantic salmon testing positive for PRV-1 or with control material. Fish were sampled at intervals to assess viral RNA transcript levels, hematocrit, erythrocytic inclusions, and histopathology. While PRV-1 replicated to high loads in all species, there was negligible mortality...
Categories: Data;
Tags: Pacific Northwest,
USGS Science Data Catalog (SDC),
Wildlife Disease,
environment
Environmental variation has important effects on host-pathogen interactions, affecting large-scale ecological processes such as the severity and frequency of epidemics. However, less is known about how the environment modulates viral fitness traits and within host infection processes. Viral genetic variation, fish host immune response and environmental parameters such as temperature have been reported to strongly influence the replication and clearance of infectious hematopoietic necrosis virus (IHNV), a significant pathogen of salmon and trout. Here, we quantified the effect of water temperature on probability of infection across time following exposure of steelhead (Oncorhynchus mykiss) to a field isolate of IHNV....
Categories: Data;
Tags: Aquatic Biology,
Pacific Northwest,
USGS Science Data Catalog (SDC),
Wildlife Disease,
aquaculture water use,
A quantitative PCR (qPCR) assay was developed to detect nuclear inclusion X (NIX) in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches.
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Alaska Coastal,
USGS Science Data Catalog (SDC),
Washington Coastal,
bacteria,
biota,
Bacterial kidney disease (BKD) caused by Renibacterium salmoninarum (Rs) occurs nearly worldwide where wild or cultured salmonid fishes are present. Control of BKD is confounded by its two modes of transmission, horizontal (fish-to-fish) and vertical (from female parent to progeny via the eggs). A highly successful BKD control strategy employed in Pacific Northwest hatcheries culturing spring Chinook salmon (Oncorhynchus tshawytscha) includes: (1) injecting pre-spawning adults with a macrolide antibiotic to improve survival and reduce Rs infection levels, (2) broodstock culling of highly infected females and (3) improved fish husbandry. However, the future availability of the injectable macrolide antibiotic (erythromycin)...
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