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Application of Environmental DNA for Inventory and Monitoring of Aquatic Species What is Environmental DNA?DNA, short for deoxyribonucleic acid, is the hereditary material in organisms that contains the biological instructions for building and maintaining them. The chemical structure of DNA is the same for all organisms, but differences exist in the order of the DNA building blocks, known as base pairs. Unique sequences of base pairs, particularly repeating patterns, provide a means to identify species, populations, and even individuals.Environmental DNA (eDNA) is nuclear or mitochondrial DNA that is released from an organism into the environment. Sources of eDNA include secreted feces, mucous, and gametes; shed skin and hair; and carcasses. eDNA can be detected in cellular or extracellular... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Document, EARTH SCIENCE > LAND SURFACE > LANDSCAPE, Federal resource managers, LCC Network Science Catalog, State agencies, All tags... Tribes, completed, environment, Fewer tags Characterizing the distribution of an endangered salmonid using environmental DNA analysis Determining species distributions accurately is crucial to developing conservation and management strategies for imperiled species, but a challenging task for small populations. We evaluated the efficacy of environmental DNA (eDNA) analysis for improving detection and thus potentially refining the known distribution of Chinook salmon (Oncorhynchus tshawytscha) in the Methow and Okanogan Subbasins of the Upper Columbia River, which span the border between Washington, USA and British Columbia, Canada. We developed an assay to target a 90 base pair sequence of Chinook DNA and used quantitative polymerase chain reaction (qPCR) to quantify the amount of Chinook eDNA in triplicate 1-L water samples collected at 48 stream... Categories: Data, Publication; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: EARTH SCIENCE > LAND SURFACE > LANDSCAPE, Federal resource managers, LCC Network Science Catalog, Publication, State agencies, All tags... Tribes, completed, environment, Fewer tags Landscape-level assessment of ESA-listed spring-chinook distribution in Upper Columbia Basin watersheds using environmental DNA The goal of this study is to use eDNA as a cost effective tool for documenting the occurrence and distribution of ESA-listed spring-chinook (Oncorhynchus tshawytscha) throughout the Okanogan and Methow watersheds in an effort to map habitat use and connectivity. Our approach will use eDNA to detect low density spring-chinook in filtered water samples to inventory distribution and re-colonization of tributaries at strategically-sampled points throughout entire stream networks. This approach will provide one of the first applications of eDNA technology for monitoring distribution of native fish species of conservation concern. The findings will help the Colville Tribe Fisheries Managers assess aquatic connectivity... Categories: Data, Project; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Basin, Data Acquisition and Development, Datasets/Database, Federal resource managers, Goal 3: Maintain hydrologic regimes, All tags... Invasive Species, LCC Network Science Catalog, Land Use Change, Methow sub-basin, Monitoring, Okanogan sub-basin, Population & Habitat Evaluation/Projection, Project, Publication, Salmon, State agencies, Training/Outreach/Workshop, Tribes, WA-1, WA-4, WA-4, WA-4, WA-4, WA-8, WA-8, WA-8, WA-8, Washington, Washington, completed, environment, Fewer tags