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Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements. Categories: Data; Tags: Columbia Environmental Research Center, climatologyMeteorologyAtmosphere, eDNA, eDNA Shedding rates, environmental DNA, All tags... mollusks, Fewer tags Food availability experiments with Actinonaias ligamentina and Lampsilis siliquoidea Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for mucket (Actinonaias ligamentina) and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing food avilability scenarios. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements. Categories: Data; Tags: Columbia Environmental Research Center, climatologyMeteorologyAtmosphere, eDNA, eDNA Shedding rates, environmental DNA, All tags... mollusks, Fewer tags Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA Data describe specificity and sensitivity testing for the L.sil2 assay. This assay is used to amplify eDNA from the freshwater mussel Lampsilis siliquoidea. Categories: Data; Tags: Aquatic Biology, Boone County, Missouri, Columbia Environmental Research Center, USGS Science Data Catalog (SDC), biota, All tags... environmental DNA, mollusks, Fewer tags Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements. Tags: Aquatic Biology, Columbia Environmental Research Center, USGS Science Data Catalog (SDC), climatologyMeteorologyAtmosphere, eDNA, All tags... eDNA Shedding rates, environmental DNA, mollusks, Fewer tags Temperature experiments with Actinonaias ligamentina and Cumberlandia monodonta Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta) and mucket (Actinonaias ligamentina) from water samples collected in the laboratory at different water temperatures. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements. Categories: Data; Tags: Columbia Environmental Research Center, climatologyMeteorologyAtmosphere, eDNA, eDNA Shedding rates, environmental DNA, All tags... mollusks, Fewer tags Laboratory estimates of eDNA degradation rates for Actinonaias ligamentina eDNA Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the mucket (Actinonaias ligamentina) from water samples collected in the laboratory. Samples were collected from experiments performed from November 01, 2019 to November 15, 2019 at the USGS Columbia Environmental Research Center, Missouri Categories: Data; Tags: Aquatic Biology, Columbia Environmental Research Center, USGS Science Data Catalog (SDC), biota, eDNA degradation, All tags... environmental DNA, mollusks, Fewer tags Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected from experiments performed from May 17, 2021 to May 30, 2021 at the USGS Columbia Environmental Research Center, Missouri Categories: Data; Tags: Aquatic Biology, Columbia Environmental Research Center, Gasconade River, USGS Science Data Catalog (SDC), biota, All tags... eDNA degradation, environmental DNA, mollusks, species detection, Fewer tags