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Data were collected in June 2016 as part of a dye-trace experiment in the Missouri River downstream from Fort Peck Dam, Montana, USA. Fluorometers were deployed at 8 measurement stations over a 135-km study reach. Approximately 51 L of Rhodamine WT (RWT) dye was injected on 26 June 2016 at 14:30 MDT. The injection occured along a transect located approximately 3000 meters downstream from the confluence of the Milk and Missouri Rivers. Injection was performed via a gravity-feed system and was complete within seven minutes of initaition. RWT dye was diluted by at least half to facilitate mixing and was injected approximately 0.30 m below the water surface. Fluorometers were placed approximately 4 to 8 m from shore...