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Climate change is a global phenomenon, but natural selection occurs within landscapes. Many global analyses predict how climate change will shape behavior and physiology, but few incorporate information from the landscape scales at which animals actually respond to selective pressure. We compared cold-water fish (redband trout Oncorhynchus mykiss newberrii) from neighboring habitats in a naturally warm, recently fragmented basin to understand how different responses to warming may arise from landscape constraints. Trout in warm, hydrologically connected Upper Klamath Lake fled summer temperatures and sought refuge in cool tributaries, while trout in an equally warm but fragmented reach of the Klamath River endured...