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This dataset includes skin temperatures of twelve Tadarida brasiliensis held in environmental chambers maintained at 7.7 (SD 0.9) °C and 91.8 (SD 0.8) % relative humidity to induce and support hibernation for up to 3 months. Bats were randomly assigned to infected and control groups at the start of the experiment and infected with conidia of Pseudogymnoascus destructans or a sham treatment (negative control). An iBBat temperature logger (Alpha Mach, Ste-Julie, Quebec, Canada) was attached to the dorsal surface of each bat and programmed to record skin temperature every 15 minutes until the end of the experiment when the bat died or was euthanized.