Use of PRD1 bacteriophage in groundwater viral transport, inactivation, and attachment studies

PRD1, an icosahedra-shaped, 62 nm (diameter), double-stranded DNA bacteriophage with an internal membrane, has emerged as an important model virus for studying the manner in which microorganisms are transported through a variety of groundwater environments. The popularity of this phage for use in transport studies involving geologic media is due, in part, to its relative stability over a range of temperatures and low degree of attachment in aquifer sediments. Laboratory and field investigations employing PRD1 are leading to a better understanding of viral attachment and transport behaviors in saturated geologic media and to improved methods for describing mathematically subsurface microbial transport at environmentally...

Categories: Publication; Types: Citation; Tags: Attachment, Groundwater, Inactivation, PRD1, Subsurface, All tags... Subsurface transport, Virus, aquifers, Fewer tags

Genetic Data Associated with Avian Keratin Disorder and Poecivirus in Black-capped Chickadees, Alaska, 2001-2017

This dataset contains results of genetic screening for Poecivirus from samples of black-capped chickadees (BCCH; Poecile atricapillus) with and without clinical signs of avian keratin disorder (AKD). Data include information on detection/non-detection of the virus in tissue collected with buccal swabs, cloacal swabs, blood samples, and fecal samples from up to 124 individuals between 2015 and 2017 from various locations in southcentral Alaska. For an additional 17 symptomatic (and one asymptomatic) individual(s) collected between 2001 and 2015 in the same region, data include measurements of viral load in beak tissue measured by qRT-PCR as well as the amount of actively replicating virus detected by 7 negative-strand...

Categories: Data; Tags: Alaska, Anchorage, Animal diseases, Animals/Vertebrates, Avian keratin disorder, All tags... Biological Science Disciplines, Biology, Biota, Birds, Black-capped chickadee, Chugiak, Disease, Disease detection, Eagle River, Ecology, Environment, Field methods, Genetics, Genetics, Genetics, Health and disease, Matanuska Valley, Natural Science Disciplines, Pathologic Processes, Perching birds, Picornavirus, Plant and animal testing, Poecivirus, Susitna Valley, USGS Science Data Catalog (SDC), Viral diseases, Virus, Viruses, Wasilla, Wildlife Biology, Wildlife Disease, Fewer tags

Comparative Virulence of Spring Viremia of Carp Virus (SVCV) Genotypes in Two Koi Varieties

Spring viremia of carp virus (SVCV, species Carp sprivivirus), is considered one of the most lethal freshwater pathogens of cyprinid fish. Common carp Cyprinus carpio L. and koi C. carpio koi are the most susceptible host fish species. The virus was formally described in the 1960's after outbreaks occurred in carp species on the European continent, but there has been a global expansion of SVCV primarily in Asia and North America. Genetic typing of the SVCV isolates separates them into four genogroups correlated with geographic origin: Ia (Asia), Ib and Ic (Eastern Europe), and Id (Central Europe). In this study we compare the virulence of eight SVCV strains including representatives of each genogroup: Ia (20040741,...

Categories: Data; Tags: Aquatic Biology, King County, Seattle, USGS Science Data Catalog (SDC), Washington, All tags... Wildlife Disease, disease, fish, koi, virus, Fewer tags

Influence of water chemistry and travel distance on bacteriophage PRD-1 transport in a sandy aquifer

Experiments were conducted to evaluate the impact of groundwater chemistry and travel distance on the transport and fate behavior of PRD-1, a bacteriophage employed as a surrogate tracer for pathogenic enteric viruses. The experiments were conducted in the unconfined aquifer at the United States Geological Survey Cape Cod Toxic-Substances Hydrology Research Site in Falmouth, Massachusetts. The transport behavior of bromide (Br−) and PRD-1 were evaluated in a sewage-effluent contaminated zone and a shallower uncontaminated zone at this site. Several multilevel sampling devices located along a 13-m transect were used to collect vertically discrete samples to examine longitudinal and vertical variability of PRD-1 retardation...

Categories: Publication; Types: Citation; Tags: Groundwater, Transport, Virus

Spring Viremia of Carp Virus (SVCV) Infection Trials of Pacific Northwest Amphibians.

Spring viremia of carp virus (SVCV) is a rhabdovirus that primarily infects cyprinid finfishes and causes a disease notifiable to the World Organization for Animal Health. Amphibians, which are sympatric with cyprinids in freshwater ecosystems, are considered non-permissive hosts of rhabdoviruses. The potential host range expansion of SVCV in an atypical host species was evaluated by testing the susceptibility of amphibians native to the Pacific Northwest. Larval long-toed salamanders Ambystoma macrodactylum and Pacific tree frog Pseudacris regilla tad-poles were exposed to SVCV strains from genotypes Ia, Ib, Ic or Id by either intraperitoneal injection, immersion, or cohabitation with virus-infected koi Cyprinus...

Categories: Data; Tags: Aquatic Biology, None, Pathogen, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... amphibian, aquatic, biota, environment, fish, virus, Fewer tags