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Use of the 5'-Nuclease Reaction for Single Nucleotide Polymorphism Genotyping in Chinook Salmon

Dates

Year
2005

Citation

Smith, Christian T., Seeb, James E., Schwenke, Piper, and Seeb, Lisa W., 2005, Use of the 5'-Nuclease Reaction for Single Nucleotide Polymorphism Genotyping in Chinook Salmon: Transactions of the American Fisheries Society, v. 134, no. 1, p. 207-217.

Summary

Migratory and stock composition studies of Chinook salmon Oncorhynchus tshawytscha require genetic markers by which a large number of individuals can be processed in a relatively short time. Given the multijurisdictional geographic range of this species, it is further desirable that genetic markers and the corresponding data be transportable across laboratories. We developed 10 single nucleotide polymorphism (SNP) genotyping assays in Chinook salmon based on the 5?-nuclease reaction. Using these assays, a single technician with two thermal cyclers can generate thousands of genotypes per day. The genotyping assays described here are easy to standardize across laboratories, and the resulting genotype data are readily combined with those [...]

Contacts

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Provenance

Data source
File Processing
File Process
Type
End Note
Reference Item
NWBLCC 20160616
Reference File
NWBLCC-20160527.xml

Additional Information

Identifiers

Type Scheme Key
DOI http://sciencebase.gov/vocab/identifierScheme 10.1577/T04-019.1; 03 10.1577/T04-019.1
ISSN http://sciencebase.gov/vocab/identifierScheme 0002-8487

Citation Extension

journalTransactions of the American Fisheries Society
parts
typeNotes
value8904
typePages
value207-217
typeVolume
value134
typeNumber
value1
citationTypeJournal Article

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