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The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
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This dataset describes the hydrogeomorphic structure and lake-tributary mixing in three intermediate-sized Lake Michigan rivermouths: Ford River, Manitowoc River, and Pere Marquette River. Data were collected from May to October 2011. Water chemistry variables were measured with a multiparameter sonde along longitudinal, lateral, and vertical transects. Magnesium, boron, and stable water isotope concentrations were also determined from grab water samples at particular depths.
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This dataset is part of the U.S. Geological Survey (USGS) Great Lakes Coastal Wetland Restoration Assessment (GLCWRA) initiative. These data represent the flowline network in the Green Bay Restoration Assessment (GBRA). It is attributed with the number of disconnections (e.g., road crossings) between the reach and Lake Ontario. The more road crossings on a flowline the more disconnected that area is from the lake and the less suitable it will be for restoration. These data help identify the condition of hydrologic separation between potential restoration areas and Lake Ontario. Low numbers represent fewer disconnections, such as culverts, between the reach and the water body requiring no flow network modification...
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During 2018, uncrewed aerial vehicles (UAVs or 'drones') were used to collect spatially referenced aerial imagery from 20 management units (sites) enrolled in the Phragmites Adaptive Management Framework, a collective learning program developed by the Great Lakes Phragmites Collaborative. Management units were located in Michigan, Ohio, and Wisconsin (USA). Invasive Phragmites australis (hereafter "Phragmites") had been managed at each management units some time previously by the landowner or land manager, and aerial imagery was then collected to create cover classifications distinguishing live and dead Phragmites from the surrounding landscape using object-based image analysis with training based on ground-truth...
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These data show grass crop and model species response to toxic chemicals (Arsenic (As)) and humic acids. Experiments were performed by collaboration between the U.S. Geological Survey, Rutgers University, and Rey Juan Carlos University. A series of individual experiments investigated beneficial effects of endophytic bacteria on grass crop growth and resilience to known plant toxicity.
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These data tables contain data collections from field experiments of Phragmites australis (ssp. australis) treated with known fungal endophytes. Tiller counts, tiller diameter, and tiller height measurements were taken every two weeks over an eight-week study period. Clones of Phragmites plants were collected from three different locations: Sandusky, Michigan; Bloomington, Indiana; and the Ottawa National Wildlife Refuge near Oak Harbor, Ohio. Additionally, data collections from a similar experiment of Phragmites australis (ssp. australis) treated with known fungal endophytes performed in a growth chamber are included. Tiller numbers and tiller heights were measured every three weeks over 15 total weeks for the...
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This is a tabular data set that includes intensity and saturation measurements of benthic invertebrates, sample residue, and preservative from samples stained with three fluorescent dyes for the development of a fluorescent staining method to enhance picking efficiency. This data set also contains data evaluating a selected fluorescent method's effects on picking efficiency and comparison to standard non-fluorescent methods for sorting benthic invertebrates.
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To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
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To determine the potential for controlling non-native Phragmites australis through use of a cut-to-drown management strategy we performed a controlled greenhouse mesocosm experiment in Ann Arbor, MI during summer 2021. Field collected Phragmites rhizomes from one site within Michigan were propagated, cuttings taken of individual stems and potted in nursery pots. Established plants were then subjected to a complete factorial combination of three submergence treatments (no submergence, partial submergence of aboveground tissues, and compete submergence of aboveground tissues) and 3 cutting treatments (no cutting, spring cutting of above ground tissues, and summer cutting of aboveground tissues). By performing weekly...
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Great Lakes coastal marshes have the potential to filter water coming off of the landscape and remove sediments and nutrients before they reach the lakes. However, this ability has largely been neutralized as vast areas of coastal wetlands have been isolated behind dikes; while at the same time the Great Lakes continue to suffer from excess phosphorus loading. In this study we used high-frequency measurements of discharge and turbidity to estimate sediment and phosphorus retention in a formerly diked coastal wetland. We found sediment and phosphorus retention to be episodic and highly related to fluctuations in water level. Low water levels in Lake Erie in late 2012 resulted in low retention in the wetland, but...
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Data represent physical (water quality, water nutrient analysis) and biological (fish, macroinvertebrate, and vegetation community) collections from the Shiawassee National Wildlife Refuge in Saginaw, MI, USA (specifically, historical management unit "Pool 1A" and the North and South management units of the Maankiki Marsh Complex.) The North and South management units of the Maankiki Marsh Complex were reconnected to the Shiawassee River in 2018, whereas Pool 1A represents a historically reconnected wetland. Additional sites within the Shiawassee River and Spaulding Drain (east of Pool 1A) were also sampled to represent parameters collected from the parent water system. All data were collected in 2019.
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These datasets represent USGS-led coastal wetland vegetation survey and mapping efforts at Metzger Marsh, part of the Ottawa National Wildlife Refuge (Ohio, USA) along the coast of western Lake Erie between 1994 and 2022. Vegetation quadrat data provide percent cover estimates per sampling quadrat and overall mean percent cover (MPC) values per species by vegetation type from 1994, and 1996-2010. Vegetation mapping (a.k.a., "photointerpretation") geospatial datasets provide full site cover visualizations and feature class information by vegetation type from 1994,1996-2002, and 2022.
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This dataset represents the locations of experimental plots that were used during the Phragmites Bioherbicide 2023 field experiment. To meet the criteria for selection, a site must have relatively dry soil with no standing water, have two or more large monotypic stands of Phragmites australis, and be easily accessible by vehicle. Experimental plots were created at these sites and one of four treatments were applied to each plot involving either tap water or bioherbicide.
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The data set includes quantitative fish abundance counts estimated from video data files collected by a Dual-frequency IDentification SONar (DIDSON) placed at the junction of a water control structure located between Pool 2B and Crane Creek (41.62133N, -83.20769W) within Ottawa National Wildlife Refuge (Lucas County, Ohio). One-hour-long video data files were used to estimate fish activity in a restored coastal wetland within Ottawa National Wildlife Refuge in the Western Basin of Lake Erie. Over 800 video hours of fish movement were recorded at the fully open fish passage structure; 136 video data files were chosen at random to represent the full video data file set and are available upon request. Fish counts from...
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These data represent the first reference genome for the invasive Phragmites australis ssp. australis (1.14 giga base pairs (Gbp)), as well as output from comparative genomic and transcriptomic analyses for invasive and native genotypes coexisting in the Great Lakes region of North America. Genome sequencing data used tillers and associated rhizome tissues collected from a single P. australis patch at the Ottawa National Wildlife Refuge near Toledo, Ohio, USA. Transcriptome analyses were produced from samples collected from three invasive and three native genotype P. australis patches from four sites around the Great Lakes in Michigan and Ohio, USA.
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Nonnative Phragmites australis (common reed) is widely distributed across North America and insufficient knowledge of P. australis has impeded the efficiency of management. To aid in Phragmites management and future studies, we used RNA-seq data from multiple types of plant tissue to construct forty-nine P. australis transcriptomes via different assembly tools and multiple parameter settings, resulting in seven assembled transcriptomes for further comprehensive assessments. The optimal transcriptome (“cd-hit”) was selected for functional annotation and downstream analyses. This data release contains the seven assembled transcriptomes, and data derived from the optimal transcriptome (functional annotations, tissue-specific...
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Plant roots have the best-understood mutualisms with microbes, but leaf and bract cell endosymbiosis have not been previously reported. Leaf and bract cells of more than 30 species in 18 families of seed plants were surveyed for the presence of intracellular bacteria and several experiments were designed to find and analyze nutrient exchanges between bacteria and plant cells. This dataset contains the results of 1) histochemical analyses to detect hormones, superoxide, and nitrogenous chemicals around bacteria within plant leaf and bract cells, 2) experiments to assess the differential absorption of isotopic nitrogen into plants, and 3) genetic analysis of bacteria isolated from plant material.
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The first basin-wide map of large stands of invasive Phragmites australis (common reed) in the coastal zone was created through a collaboration between the U.S. Geological Survey and Michigan Tech Research Institute (Bourgeau-Chavez et al 2013). This data set represents a revised version of that map and was created using multi-temporal PALSAR data and Landsat images from 2016-2017. In addition to Phragmites distribution, the data sets shows several land cover types including urban, agriculture, forest, shrub, emergent wetland, forested wetland, and some based on the dominant plant species (e.g., Schoenoplectus, Typha). The classified map was validated using over 400 field visits.
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The first basin-wide map of large stands of invasive Phragmites australis (common reed) in the coastal zone was created through a collaboration between the U.S. Geological Survey and Michigan Tech Research Institute (Bourgeau-Chavez et al 2013). This data set represents a revised version of that map and was created using multi-temporal PALSAR data and Landsat images from 2016-2017. In addition to Phragmites distribution, the data sets shows several land cover types including urban, agriculture, forest, shrub, emergent wetland, forested wetland, and some based on the dominant plant species (e.g., Schoenoplectus, Typha). The classified map was validated using over 400 field visits.This map covers the Southern portion...
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This tabular data set contains estimates of total phosphorus concentration and mass, as well as measurements of water discharge and volume at a diked wetland pool (Pool 2B) that was reconnected to a Lake Erie tributary (Crane Creek) via a water control structure in March 2011. Sampling occurred during 2013 and 2014. In addition, this data set contains evaluations of movement of total phosphorus and water through the reconnecting structure, frequency of changes in flow direction through the reconnecting structure, and dissolved oxygen measurements in the reconnected wetland pool.


map background search result map search result map Development of fluorescent staining method and effect of fluorescent staining on benthic invertebrate picking efficiency Total phosphorus and water flux at a restored hydrologic connection at Ottawa National Wildlife Refuge in 2013 and 2014 Land cover map including wetlands and invasive Phragmites circa 2017 Land cover map including wetlands and invasive Phragmites circa 2017 for Central Lake Erie Using turbidity measurements to estimate phosphorus and sediment flux in a Great Lakes Coastal Marsh, in Ohio Hydrogeochemical Mixing data from Lake Michigan Tributaries 2011 DIDSON video collection of Coastal Lake Erie Wetland, Lucas Co, Ohio in 2011 The effects of North American fungi and bacteria on Phragmites australis leaves 2017-2019, with comparisons to the global Phragmites microbiome Physical and Biological Monitoring Data Collected from Restored Wetland Units at Shiawassee National Wildlife Refuge, Saginaw, MI, USA (2019) Data collected to support research on grass crop growth promotion and biostimulation by endophytic bacteria Phragmites australis responses to and microbial community composition of greenhouse soils (2018-2019 experiment) Land cover classifications and associated data from treatment areas enrolled in the Phragmites Adaptive Management Framework, 2018 Reference genome for Phragmites australis (Poaceae, subfamily Arundinoideae) and comparison of North American invasive genotype (ssp. australis) and native (ssp. americanus) Effects of fungal endophytes on invasive Phragmites australis (ssp. australis) performance in growth chamber and field experiments Phragmites australis Transcriptome Assembly Optimization Histochemical study of nitrogen-transfer endosymbiosis 2021 USGS Phragmites australis Greenhouse Submergence Experiment Data conducted in Ann Arbor, MI Great Lakes Coastal Wetland Restoration Assessment (GLCWRA) Green Bay, U.S.: Degree Flowlines Vegetation survey and photointerpretation data for Metzger Marsh, OH, USA (1994-2022) Field Site Locations from Phragmites Bioherbicide 2023 Field Experiment in Southeast Michigan Development of fluorescent staining method and effect of fluorescent staining on benthic invertebrate picking efficiency Phragmites australis Transcriptome Assembly Optimization Vegetation survey and photointerpretation data for Metzger Marsh, OH, USA (1994-2022) Physical and Biological Monitoring Data Collected from Restored Wetland Units at Shiawassee National Wildlife Refuge, Saginaw, MI, USA (2019) Total phosphorus and water flux at a restored hydrologic connection at Ottawa National Wildlife Refuge in 2013 and 2014 DIDSON video collection of Coastal Lake Erie Wetland, Lucas Co, Ohio in 2011 2021 USGS Phragmites australis Greenhouse Submergence Experiment Data conducted in Ann Arbor, MI Using turbidity measurements to estimate phosphorus and sediment flux in a Great Lakes Coastal Marsh, in Ohio Field Site Locations from Phragmites Bioherbicide 2023 Field Experiment in Southeast Michigan Land cover map including wetlands and invasive Phragmites circa 2017 for Central Lake Erie Hydrogeochemical Mixing data from Lake Michigan Tributaries 2011 Phragmites australis responses to and microbial community composition of greenhouse soils (2018-2019 experiment) Great Lakes Coastal Wetland Restoration Assessment (GLCWRA) Green Bay, U.S.: Degree Flowlines Land cover classifications and associated data from treatment areas enrolled in the Phragmites Adaptive Management Framework, 2018 Land cover map including wetlands and invasive Phragmites circa 2017 Reference genome for Phragmites australis (Poaceae, subfamily Arundinoideae) and comparison of North American invasive genotype (ssp. australis) and native (ssp. americanus) Effects of fungal endophytes on invasive Phragmites australis (ssp. australis) performance in growth chamber and field experiments Histochemical study of nitrogen-transfer endosymbiosis Data collected to support research on grass crop growth promotion and biostimulation by endophytic bacteria The effects of North American fungi and bacteria on Phragmites australis leaves 2017-2019, with comparisons to the global Phragmites microbiome