Filters: Categories: Data (X) > partyWithName: Kurt P Kowalski (X)
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The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
This dataset describes the hydrogeomorphic structure and lake-tributary mixing in three intermediate-sized Lake Michigan rivermouths: Ford River, Manitowoc River, and Pere Marquette River. Data were collected from May to October 2011. Water chemistry variables were measured with a multiparameter sonde along longitudinal, lateral, and vertical transects. Magnesium, boron, and stable water isotope concentrations were also determined from grab water samples at particular depths.
During 2018, uncrewed aerial vehicles (UAVs or 'drones') were used to collect spatially referenced aerial imagery from 20 management units (sites) enrolled in the Phragmites Adaptive Management Framework, a collective learning program developed by the Great Lakes Phragmites Collaborative. Management units were located in Michigan, Ohio, and Wisconsin (USA). Invasive Phragmites australis (hereafter "Phragmites") had been managed at each management units some time previously by the landowner or land manager, and aerial imagery was then collected to create cover classifications distinguishing live and dead Phragmites from the surrounding landscape using object-based image analysis with training based on ground-truth...
These data show grass crop and model species response to toxic chemicals (Arsenic (As)) and humic acids. Experiments were performed by collaboration between the U.S. Geological Survey, Rutgers University, and Rey Juan Carlos University. A series of individual experiments investigated beneficial effects of endophytic bacteria on grass crop growth and resilience to known plant toxicity.
Categories: Data;
Tags: Ecology,
Genetics,
North America,
USGS Science Data Catalog (SDC),
bacteria,
These data tables contain data collections from field experiments of Phragmites australis (ssp. australis) treated with known fungal endophytes. Tiller counts, tiller diameter, and tiller height measurements were taken every two weeks over an eight-week study period. Clones of Phragmites plants were collected from three different locations: Sandusky, Michigan; Bloomington, Indiana; and the Ottawa National Wildlife Refuge near Oak Harbor, Ohio. Additionally, data collections from a similar experiment of Phragmites australis (ssp. australis) treated with known fungal endophytes performed in a growth chamber are included. Tiller numbers and tiller heights were measured every three weeks over 15 total weeks for the...
Categories: Data;
Tags: Aquatic Biology,
Bloomington,
Ecology,
Indiana,
Indiana University Research and Teaching Preserve,
This is a tabular data set that includes intensity and saturation measurements of benthic invertebrates, sample residue, and preservative from samples stained with three fluorescent dyes for the development of a fluorescent staining method to enhance picking efficiency. This data set also contains data evaluating a selected fluorescent method's effects on picking efficiency and comparison to standard non-fluorescent methods for sorting benthic invertebrates.
Categories: Data;
Types: Citation;
Tags: Michigan,
USGS Science Data Catalog (SDC),
Washtenaw,
benthos,
environment,
This study area consists of a 10-km inland buffer of the U. S. Great Lakes shoreline. Islands within the lakes were included in this invasive Phragmites mapping project where remotely sensed imagery scenes were available.
Categories: Data,
Map;
Types: Map Service,
OGC WMS Layer;
Tags: Accountability,
GLRI,
Great Lakes,
Great Lakes Restoration Initiative,
Invasive Species,
To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
To determine the potential for controlling non-native Phragmites australis through use of a cut-to-drown management strategy we performed a controlled greenhouse mesocosm experiment in Ann Arbor, MI during summer 2021. Field collected Phragmites rhizomes from one site within Michigan were propagated, cuttings taken of individual stems and potted in nursery pots. Established plants were then subjected to a complete factorial combination of three submergence treatments (no submergence, partial submergence of aboveground tissues, and compete submergence of aboveground tissues) and 3 cutting treatments (no cutting, spring cutting of above ground tissues, and summer cutting of aboveground tissues). By performing weekly...
Categories: Data;
Tags: Ann Arbor East,
Aquatic Biology,
Ecology,
Michigan,
USGS Science Data Catalog (SDC),
Great Lakes coastal marshes have the potential to filter water coming off of the landscape and remove sediments and nutrients before they reach the lakes. However, this ability has largely been neutralized as vast areas of coastal wetlands have been isolated behind dikes; while at the same time the Great Lakes continue to suffer from excess phosphorus loading. In this study we used high-frequency measurements of discharge and turbidity to estimate sediment and phosphorus retention in a formerly diked coastal wetland. We found sediment and phosphorus retention to be episodic and highly related to fluctuations in water level. Low water levels in Lake Erie in late 2012 resulted in low retention in the wetland, but...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Hydrology,
Hydrology,
Lake Erie,
Ottawa National Wildlife Refuge, Lucas County, Ohio,
Phosphorus,
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
Data represent physical (water quality, water nutrient analysis) and biological (fish, macroinvertebrate, and vegetation community) collections from the Shiawassee National Wildlife Refuge in Saginaw, MI, USA (specifically, historical management unit "Pool 1A" and the North and South management units of the Maankiki Marsh Complex.) The North and South management units of the Maankiki Marsh Complex were reconnected to the Shiawassee River in 2018, whereas Pool 1A represents a historically reconnected wetland. Additional sites within the Shiawassee River and Spaulding Drain (east of Pool 1A) were also sampled to represent parameters collected from the parent water system. All data were collected in 2019.
These datasets represent USGS-led coastal wetland vegetation survey and mapping efforts at Metzger Marsh, part of the Ottawa National Wildlife Refuge (Ohio, USA) along the coast of western Lake Erie between 1994 and 2022. Vegetation quadrat data provide percent cover estimates per sampling quadrat and overall mean percent cover (MPC) values per species by vegetation type from 1994, and 1996-2010. Vegetation mapping (a.k.a., "photointerpretation") geospatial datasets provide full site cover visualizations and feature class information by vegetation type from 1994,1996-2002, and 2022.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Hydrology,
USGS Science Data Catalog (SDC),
Upper Ohio,
The data set includes quantitative fish abundance counts estimated from video data files collected by a Dual-frequency IDentification SONar (DIDSON) placed at the junction of a water control structure located between Pool 2B and Crane Creek (41.62133N, -83.20769W) within Ottawa National Wildlife Refuge (Lucas County, Ohio). One-hour-long video data files were used to estimate fish activity in a restored coastal wetland within Ottawa National Wildlife Refuge in the Western Basin of Lake Erie. Over 800 video hours of fish movement were recorded at the fully open fish passage structure; 136 video data files were chosen at random to represent the full video data file set and are available upon request. Fish counts from...
These data represent the first reference genome for the invasive Phragmites australis ssp. australis (1.14 giga base pairs (Gbp)), as well as output from comparative genomic and transcriptomic analyses for invasive and native genotypes coexisting in the Great Lakes region of North America. Genome sequencing data used tillers and associated rhizome tissues collected from a single P. australis patch at the Ottawa National Wildlife Refuge near Toledo, Ohio, USA. Transcriptome analyses were produced from samples collected from three invasive and three native genotype P. australis patches from four sites around the Great Lakes in Michigan and Ohio, USA.
Maps of areas greater than 0.2 hectare (0.5 acre) dominated by invasive Phragmites australis were created for the coastal region (shoreline to 10 km inland) of the United States side the Great Lakes and connecting water ways. The maps were developed using unsupervised/supervised classification methods and ground truth data collected during 2010 and 2011 in conjunction with multi-season ALOS PALSAR imagery (for the remote sensing-based iterative classification process), as well as through the interpretation of aerial photography to reduce classification confusion. Overall classification accuracy compared to field data for mapping was approximately 86%.
Categories: Data,
Map;
Types: Map Service,
OGC WMS Layer;
Tags: Accountability,
GLRI,
Great Lakes,
Great Lakes Restoration Initiative,
Invasive Species,
This network of inland streams, wetlands and water bodies is a composite of two layers from the National Hydrography Dataset Plus (NHD+ flow lines and water bodies), and all available wetlands from the National Wetlands Inventory (NWI) and Wisconsin Wetlands Inventory (WWI). In combination, these layers provide a network template of inland corridors for assessing relative vulnerability to future invasions of Phragmites.
Categories: Data,
Map;
Types: Map Service,
OGC WMS Layer;
Tags: Accountability,
GLRI,
Great Lakes,
Great Lakes Restoration Initiative,
Invasive Species,
Nonnative Phragmites australis (common reed) is widely distributed across North America and insufficient knowledge of P. australis has impeded the efficiency of management. To aid in Phragmites management and future studies, we used RNA-seq data from multiple types of plant tissue to construct forty-nine P. australis transcriptomes via different assembly tools and multiple parameter settings, resulting in seven assembled transcriptomes for further comprehensive assessments. The optimal transcriptome (“cd-hit”) was selected for functional annotation and downstream analyses. This data release contains the seven assembled transcriptomes, and data derived from the optimal transcriptome (functional annotations, tissue-specific...
Plant roots have the best-understood mutualisms with microbes, but leaf and bract cell endosymbiosis have not been previously reported. Leaf and bract cells of more than 30 species in 18 families of seed plants were surveyed for the presence of intracellular bacteria and several experiments were designed to find and analyze nutrient exchanges between bacteria and plant cells. This dataset contains the results of 1) histochemical analyses to detect hormones, superoxide, and nitrogenous chemicals around bacteria within plant leaf and bract cells, 2) experiments to assess the differential absorption of isotopic nitrogen into plants, and 3) genetic analysis of bacteria isolated from plant material.
These data represent coastal corridors exposed by lake levels reduced from mean 2009 water surface elevations. These elevations were established by values published by the United States Army Corps of Engineers (USACE), and based on a network of multiple gages within each lake. The corridors were derived from two data sources: 5-m resolution lidar-based topo-bathymetry produced by the USACE Joint Airborne Lidar-Based Technical Center of eXpertise (JALBTCX) and the National Oceanic and Atmospheric Administration (NOAA) Coastal Services Center, and bathymetric contour lines produced by the NOAA Great Lakes Environmental Research Laboratory (GLERL). The JALBTCX lidar-based topo-bathymetry were used to produce representations...
Categories: Data,
Map;
Types: Map Service,
OGC WMS Layer;
Tags: Accountability,
GLERL bathymetry,
GLRI,
Great Lakes,
Great Lakes Restoration Initiative,
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