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Improving molecular techniques for monitoring Asian carps: Characterizing eDNA associated with spawning, eDNA persistence, and validation of new marker technology Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and... Categories: Project; Tags: Asian Carp, GLRI, Great Lakes Restoration Initiative, Great Lakes Restoration Initiative, Invasive Species, All tags... Template 66, Template 673, Fewer tags Improving molecular techniques for detecting Asian carps: Using LAMP technology to make it portable and faster Description of Work The field portion of a method validation study was completed August 2015. The study was designed to test the accuracy of a LAMP method to detect eDNA of Asian carps during simulated fish transport. During the study, more than 10,000 minnows were held in each of three fish transport tanks. Ten 4-inch silver carp were placed in one tank, a single 4-inch silver carp in the second tank while the third tank had no silver carp and served as a negative control. The LAMP method used amplifies the DNA of both bighead carp and silver carp. Three replicate trials were completed on separate days with side-by-side analyses completed by three novice and two expert users. Expert users had extensive experience... Categories: Project; Tags: GLRI, Great Lakes Restoration Initiative, Invasive Species, Template 26 Targeted Delivery of Control Agents to Bigheaded Carps Description of Work Studies assessing seasonal and spatial changes in digestive enzymes and gill raker morphology in bigheaded carps and native planktivorous fishes have been completed. Results indicate that bigheaded carps feed earlier in the year than native filter-feeding fishes and that certain digestive enzymes present in bigheaded carps are either not present in some native fishes or are much less active in the native species than in bigheaded carps. Results also indicate that the gill raker morphology of bigheaded carps is relatively constant with minimal seasonally or spatially relevant changes unlike that of the native gizzard shad (Dorosoma cepedianum) which had both seasonal and spatially correlated changes... Categories: Project; Tags: Asian Carp, GLRI, Great Lakes, Great Lakes Restoration Initiative, Great Lakes Restoration Initiative, All tags... Invasive Species, Template 66, Template 673, Fewer tags