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Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. Types: Citation; Tags: Aquatic Biology, California, Genetics, Idaho, Montana, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Wildlife Disease, Wyoming, environmental DNA, molecular biology, parasites, Fewer tags Climate-Induced Expansions of Invasive Species in the Pacific Northwest, North America: a Synthesis of Observations and Projections Climate change may facilitate the expansion of non-native invasive species (NIS) in aquatic and terrestrial systems. However, empirical evidence remains scarce and poorly synthesized at scales necessary for effective management. We conducted a literature synthesis to assess the state of research on the observed and predicted effects of climate change on a suite of 398 aquatic and terrestrial NIS now present in or a major threat to aquatic and terrestrial ecosystems of the Pacific Northwest (PNW), USA and British Columbia. Surprisingly, very few studies (n = 15) have investigated the observed effects of climate change on the distribution, abundance, spread, or impact of the focal NIS, with only five studies focusing... Categories: Publication; Types: Citation; Tags: Fish, Northwest CASC, Other Wildlife, Plants, Science Tools For Managers, All tags... State of the Science, Wildlife and Plants, Fewer tags A Framework to Integrate Innovations in Invasion Science for Proactive Management Invasive alien species (IAS) are a rising threat to biodiversity, national security, and regional economies, with impacts in the hundreds of billions of U.S. dollars annually. Proactive or predictive approaches guided by scientific knowledge are essential to keeping pace with growing impacts of invasions under climate change. Although the rapid development of diverse technologies and approaches has produced tools with the potential to greatly accelerate invasion research and management, innovation has far outpaced implementation and coordination. Technological and methodological syntheses are urgently needed to close the growing implementation gap and facilitate interdisciplinary collaboration and synergy among... Categories: Publication; Types: Citation Yellowstone Cutthroat Trout and Brown Trout capture-recapture and diet data from Duck Creek, MT in summer and fall (2013-2016) We collected lengths and weights and diets of Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and brown trout (Salmo trutta) above (Yellowstone cutthroat trout allopatry) and below (Yellowstone cutthroat trout sympatry with brown trout) a natural barrier in Duck Creek, Montana. Types: Citation; Tags: Big Timber, Montana, USGS Science Data Catalog (SDC), Upper Yellowstone, Wildlife Biology, All tags... biota, fisheries, freshwater ecosystems, Fewer tags