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Lampricide concentrations in larval sea lamprey (Petromyzon marinus) carcasses collected following two TFM and two TFM/Bayluscide treatments were determined to support risk assessments for non-target organisms that may consume lampricide-laden carcasses. Carcasses were removed from the treated streams either immediately after completion of the treatment or 2-days post mortem. The estimated average TFM concentration in the fresh carcasses (n = 80) collected from the rivers was 4.58 µg·g-1 (SEM =1.06 µg·g-1). The average concentration of niclosamide (the active ingredient in Bayluscide) in the fresh carcasses from the two rivers treated with TFM and Bayluscide was 0.492 µg·g-1 (SEM = 0.210 µg·g-1). The mean 2-day...
## Descriptions for each of the code files are listed below. ## 1) Adult SL density The file “Adult_density_Rscript_code_release.R” contains R code for running linear mixed models of log-transformed copy numbers using tank as the random effect and density as the fixed effect (log10(copies) ~ log10(density) + (1|Tank)). Required data includes: tank ID, density of sea lamprey in the tank, and copy numbers detected in the qPCR assays. Outputs include: The mean copy numbers, log transformed mean copy numbers, slope of the equation for the linear mixed models, p-values. ## 2) Larval SL density The file “larval_density_Rscript_IPDS.R” contains R code for running a linear model to looks for trends between copy numbers...
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This study builds upon work conducted under the Great Lakes Fishery Commission (GLFC) Technical Assistance Program that was funded to prepare and evaluate an alternative solid formulation of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) in a laboratory flume. The project aligns within the lampricide theme in the Sea Lamprey Research Program by improving lampricide performance, efficacy, and safety through the development of a more environmentally compatible lampricide formulation compared to the current surfactant-based TFM bar. The study involved performing field applications of experimental TFM pellets manufactured by collaborators at Northland College (Ashland, WI). Applications were conducted on small...
Six R code files were used to analyze data for the study entitled: Distribution of Niclosamide Following Granular Bayer Applications in Lentic Environments. The R program files included: code to summarize the distribution of niclosamide in the water column outside of the studied treatment plot. code to summarize the distribution of niclosamide in the water column inside of the studied treatment plot. code to summarize the distribution of niclosamide in the sediment inside of the studied treatment plot code to summarize the depth, temperature and pH of the water column inside of the studied treatment plot code to summarize the temperature of the sediment inside of the studied treatment plot pre and post granular...
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AEH-18-Baybar-01_compiled_for_R: CSV file with column headers; Trial Date (date the individual trial took place), Location of SL (where they ended up in the system at the end of trial), Total SL Mort (how many dead SL were found in this location), Total SL (how many SL were found in this location), Treatment type (Control, TFM, or Nic), Mainstream chemical (chem that was in mainstream), Side Channel/Seep Chemical (chemical that was in the avoidance areas of the stream) AEH-18-Baybar-Nic-concentrations: CSV file with Date of each trial, trial type (Nic, TFM, or Control), The 7 sample locations in the system, and Nic concentrations (ppb) for each hour at each location. Also included is the mean, std dev, min, and...
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The granular formulation of Bayluscide [Bayluscide 3.2% Granular Sea Lamprey Larvicide, granular Bayluscide (gB)] is applied in lentic and lotic systems to survey (assessment) and kill (treatment) larval sea lampreys (Petromyzon marinus) in the Great Lakes basin. Granules are spread on the water surface, settle to the sediment surface, and dissolve. The potential risk of niclosamide exposure [5 Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxybenzamide], the active ingredient of gB, to non-target organisms located downstream of survey plots, is a concern of partner agencies (State-level Natural Resource Departments, U.S. Fish and Wildlife Service’s Ecological Service, Fisheries and Oceans Canada Species at Risk Branch)....
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This study continues our investigations into the effects of lampricides on mussels by extending research into potential effects on behavioral and reproductive endpoints on the mussel plain pocketbook (Lampsilis cardium). We hypothesized that TFM and TFM:NIC would reduce the viability of free glochidia in a dose-dependent manner, that older glochidia would be more sensitive than younger glochidia, and that exposure of gravid females to TFM and TFM:NIC would cause a suite of behavioral and reproductive effects. These data could be important in documenting the presence (or absence) of potential behavioral and reproductive effects of lampricides on mussels and could inform management decisions. For example, these data...
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Bayluscide, the 2-aminoethanol salt of niclosamide (2′, 5-dichloro-4′-nitrosalicylanilide), is a pesticide that is used in conjunction with TFM (4-Nitro-3-(trifluoromethyl)phenol) to treat streams tributary to the Great Lakes that are infested with invasive parasitic sea lamprey (Petromyzon marinus). The application TFM with 0.5 to 2.0 percent (%) Bayluscide can significantly reduce the amount of TFM required to achieve effective control. The current formulation of Bayluscide used in combination TFM during stream treatments conducted the Great Lakes Fishery Commission binational Sea Lamprey Control Program is an emulsifiable concentrate (EC). The Bayluscide EC formulation is highly effective; however, it degrades...
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Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with of 4-Nitro-3-(trifluoromethyl)phenol (TFM, a lampricide) is essential to reducing the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of treating streams with lampricide is ensuring that the proper amount of TFM is applied, effectively controlling sea lamprey populations while minimizing effects on non-target species. In this study, the Trout, Ocqueoc and Sucker Rivers, were selected to complete replicated stream-side bioassays in May, July, and September 2021 to determine seasonal changes in sensitivity to TFM. Larvae were collected and bioassays were...
Categories: Data; Tags: Michigan, Great Lakes, biota
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Temporal and spatial distribution of niclosamide in the water column and sediment were evaluated after the application of granular Bayluscide in six lentic Sea Lamprey (Petromyzon marinus) larval assessment plots. Water and sediment were collected 0.25, 1, 3, 5, and 7 hours after application and were analyzed for niclosamide, the active ingredient in granular Bayluscide. Water samples were collected from five heights in the water column (1 cm, 13 cm, 26 cm, ½ water column, and water surface) at five locations inside and four locations 10 m outside of each assessment plot. Sediment was collected from 18 locations within each plot. Niclosamide water concentrations inside and outside of the plots did not vary by depth...
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The dataset was generated to verify the suitability of the new TFM bar formulation for wide-scale use in the Sea Lamprey Control Program (SLCP). Dataset includes TFM concentrations of three streams (each used twice) where randomized experimental applications of new and old TFM bar formulation dissolution trials were conducted, TFM concentrations of 12 laboratory flume experimental applications of new and old TFM bar formulation dissolution trials, various collected water chemistry parameters, % Active ingredient data from both new and old TFM bar formulation under normal storage and elevated temperature storage conditions, and bar hardness data from both new and old TFM bar formulation under normal storage and elevated...
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Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with 4-nitro-3-(trifluoromethyl)phenol (TFM, a pesticide) stream treatments reduces the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of stream treatments is ensuring that the proper amount of TFM is applied that will effectively control sea lamprey populations and minimize the effects on non-target species. In this study, replicated streamside bioassays were conducted in May, July, and September at six sea lamprey infested rivers in Michigan to determine potential seasonal changes in sensitivity of lamprey to TFM. Larvae (≥ 60 mm) were collected with backpack...
The data set is six separate csv files. Four of which contain the quantity of DNA copy numbers and fluor used to analyze the DNA quantities collected from water samples from four separate portions of the study (adult SL field, adult SL lab, larval SL field, larval SL lab) and need to be in their own csv file. Also included is a csv with adult SL trapping data, a csv for larval SL shocking data, and a csv with the volume that was filtered for our DNA extractions.
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This study continues our investigations into the effects of lampricides on mussels by extending research into potential effects on behavioral and reproductive endpoints on the plain pocketbook mussel(Lampsilis cardium). We hypothesized that TFM (3-trifluoromethyl-4'-nitrophenol) and TFM with Niclosamide (NIC, 2', 5-dichloro-4'-nitrosalicylanilide, hereafter TFM:NIC) would reduce the viability of free glochidia in a dose-dependent manner, that older glochidia would be more sensitive than younger glochidia, and that exposure of gravid females to TFM and TFM:NIC would cause a suite of behavioral and reproductive effects. These data could be important in documenting the presence (or absence) of potential behavioral...


    map background search result map search result map Distribution of Niclosamide Following Granular Bayer Applications in Lentic Environments data Use of an artificial stream to monitor avoidancebehavior of larval sea lamprey in response to TFM and Niclosamide Field Evaluation of an Improved Solid TFM Formulation for Use in Treating Small Tributary Streams, data Determination of the seasonality effect on sea lamprey and TFM efficacy – Year One (data) Behavioral and Reproductive Effects of the Lampricides TFM and TFM:1% Niclosamide on Native Freshwater Mussels - Data Release Data Release for Distribution of Niclosamide Following Granular Bayluscide Applications in Lotic Systems Behavioral and Reproductive Effects of the Lampricides TFM and TFM:1% Niclosamide on Native Freshwater Mussels - SPSS Code Release Data Release for Laboratory and field comparisons of TFM bar formulations used to treat small streams for larval sea lamprey Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Data and Code Release: Comparative toxicity of the Bayluscide EC and SC formulations Field Evaluation of an Improved Solid TFM Formulation for Use in Treating Small Tributary Streams, data Determination of the seasonality effect on sea lamprey and TFM efficacy – Year One (data) Data Release for Laboratory and field comparisons of TFM bar formulations used to treat small streams for larval sea lamprey Data and Code Release: Determination of Larval Sea Lamprey (Petromyzon marinus) Seasonal Sensitivity Differences to Lampricide Treatment Behavioral and Reproductive Effects of the Lampricides TFM and TFM:1% Niclosamide on Native Freshwater Mussels - Data Release Behavioral and Reproductive Effects of the Lampricides TFM and TFM:1% Niclosamide on Native Freshwater Mussels - SPSS Code Release Data Release for Distribution of Niclosamide Following Granular Bayluscide Applications in Lotic Systems Data and Code Release: Comparative toxicity of the Bayluscide EC and SC formulations Use of an artificial stream to monitor avoidancebehavior of larval sea lamprey in response to TFM and Niclosamide Distribution of Niclosamide Following Granular Bayer Applications in Lentic Environments data