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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Poor survival, growth and reproduction of the amphipod Hyalella azteca has been observed in exposures using reconstituted waters. Previous studies have reported success in H. azteca water-only exposures with the addition of relatively high concentrations of bromide (Br). This study evaluated the influence of lower environmentally representative concentrations of Br on the response of the amphipod H. azteca in 42-d water-only exposure conditions in various reconstituted waters. Improved performance of H. azteca was observed in reconstituted waters with >0.02 mg Br/L. These tables and figures provide online supplemental information that will not be included in the journal article.
Categories: Data;
Types: Citation;
Tags: NA,
chronic testing,
methods development,
water-only testing
The vernal pool fairy shrimp, Branchinecta lynchi, (Branchiopoda; Anostraca) and other fairy shrimp species have been listed as ‘threatened’ or ‘endangered’ under the United States’ Endangered Species Act (ESA). There is little information about the sensitivity of Branchinecta spp. to toxic effects of contaminants, making it difficult to determine whether they are adequately protected by water quality criteria. A series of acute (24 hr) lethality/immobilization tests were conducted with three species of fairy shrimp (Branchinecta lynchi, Branchinecta lindahli, and Thamnocephalus platyurus) and 10 chemicals with varying modes of toxic action: ammonia, potassium, chloride, sulfate, chromium(VI), copper, nickel, zinc,...
Categories: Data;
Types: Citation;
Tags: NA,
USGS Science Data Catalog (SDC),
acute toxicity,
vernal pool fairy shrimp,
water-only toxicity
Studies have been conducted to refine USEPA, ASTM, and Environment Canada standard methods for conducting 42-d reproduction tests with H. azteca in water or in sediment. Modifications to the H. azteca method include better defined ionic composition requirements for exposure water (i.e., >15 mg/L of chloride and >0.02 mg/L of bromide) and improved survival, growth and reproduction with alternate diets provided as increased rations over time in water-only or whole-sediment toxicity tests. A total of 24 laboratories volunteered to participate in an inter-laboratory study evaluating the performance of H. azteca in 42-d studies in control sand or control sediment using the refined methods. Improved growth and reproduction...
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