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In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well...
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Laboratory analysis of innate American kestrel, Falco sparverius, immune response after exposure to flame retardant, isopropylphenyl phosphate (ITP) through 21 days post hatch. Data consist of flow cytometry files that were generated in the analysis of white blood cells from kestrel blood. Thus, data are in standard format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.
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Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate...
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard...
    map background search result map search result map Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016 Xenopus metamorphosis after neonicotinoid exposure Laboratory analysis assessing immune response after flame retardant exposure in American kestrels, Falco sparverius, through 21 days post-hatch Ploidy and genome size estimates of artificially produced larval pallid sturgeon, Scaphirhynchus albus Microchemistry demographics and development data from wild caught black carp in the Mississippi River basin, 2011-18 Ploidy and genome size estimates of artificially produced larval pallid sturgeon, Scaphirhynchus albus Xenopus metamorphosis after neonicotinoid exposure Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016 Microchemistry demographics and development data from wild caught black carp in the Mississippi River basin, 2011-18 Laboratory analysis assessing immune response after flame retardant exposure in American kestrels, Falco sparverius, through 21 days post-hatch