Filters: Tags: {"type":"Harvest Set"} (X) > partyWithName: Denise M Akob (X)
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Acetylene fermentation assays, nitrogen fixation assays, and growth studies were performed with Pelobacter sp. strain SFB93 and Pelobacter acetylenicus DSM3246. Data includes concentrations of acetylene and ethylene over time, and growth measured with OD680 and cell counts.
Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but...
Categories: Data;
Tags: Barnstable County,
Cape Cod,
DNA sequencing,
Environmental Health,
Hydrology,
Data supporting the establishment of Syntrophotalea acetylenivorans SFB93 as a new microbial species. Data release includes growth curve and preferred growth conditions for the species.
Categories: Data;
Tags: Aquatic Biology,
Environmental Health,
USGS Science Data Catalog (SDC),
Water Quality,
Water Resources,
Acetylene (C2H2) is a molecule rarely found in nature, with few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs, however we hypothesized that there may be yet unrecognized diversity of acetylenotrophs in nature. In this study, we expanded this diversity by isolating an aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethene (TCE)-contaminated soils undergoing bioremediation. TCE-contaminated soils from the NASA Ames Research Center in California were used to establish soil microcosms with acetylene as the primary carbon substrate and acetylene...
Data supporting "Common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities", describing iron reduction experiments, microbial community profiling, and statistical testing of data.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Energy Resources,
Environmental Health,
Fayette County,
Oil and gas (OG) wastewaters are commonly disposed of by underground injection and previous research showed that activities at a disposal facility in West Virginia affected stream biogeochemistry and sediment microbial communities downstream from the facility. Microorganisms can control the fate and transport of organic and inorganic components of OG wastewater highlighting the need to characterize the effects of OG wastewater components on microbial activity. We conducted a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and hydraulic fracturing fluid additives (2,2-dibromo-3-nitrilopropionamide (DBNPA), a biocide, and ethylene glycol, an anti-scaling additive),...
Categories: Data;
Tags: Biochemistry,
DNA sequencing,
Energy Resources,
Environmental Health,
Fayette County,
The Permian Basin, straddling New Mexico and Texas, is one of the most productive oil and gas (OG) provinces in the United States. OG production yields large volumes of wastewater that contain elevated concentrations of major ions including salts (also referred to as brines), and trace organic and inorganic constituents. These OG wastewaters pose unknown environmental health risks, particularly in the case of accidental or intentional releases. Releases of OG wastewaters have resulted in water-quality and environmental health effects at sites in West Virginia (Akob, et al., 2016, Orem et al. 2017, Kassotis et al. 2016) and in the Williston Basin region in Montana and North Dakota (Cozzarelli et al. 2017, Cozzarelli...
Water, sediment, anuran food (leaf litter and detritus) and whole body larval amphibian composites were analyzed for over 100 current-use pesticides from 24 wetlands representing land-use gradients in the New Jersey Pinelands, USA during 2014-2016. Emerging amphibian pathogens, Ranavirus and Batrachochytrium dendrobatidis (Bd) were also assessed in individual larvae at the 24 wetlands. Tables in this data release contain the results from these analyses, with quality assurance summaries, along with site information, including land-use classifications.
Categories: Data;
Tags: Aquatic Biology,
New Jersey,
Pinelands,
USGS Science Data Catalog (SDC),
Water Quality,
Water chemistry data for samples collected in 2016 in the Wolf Creek and Buffalo Creek watersheds in West Virginia. The dataset includes stream characteristics including flow and location information for sampling sites.
Categories: Data;
Tags: Environmental Health,
USGS Science Data Catalog (SDC),
United States,
Water Quality,
West Virginia,
This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by...
Categories: Data;
Tags: Environmental Health,
Trenton West,
USGS Science Data Catalog (SDC),
Water Resources,
aquatic biology,
This USGS data release includes all the data presented in peer-reviewed publication entitled "Degradation of MCHM (4-methylcyclohexanemethanol) in Sediments from Elk River, West Virginia". We conducted experiments on crude MCHM to examine photooxidation or biodegradation. We also assessed the potential of sediments to serve as a long-term source of MCHM and well as the potential for native microbial communities to catalyze the anaerobic breakdown of MCHM. We developed a quantitative method to measure the 2 isomers (cis- and trans-) of 4-MCHM, using solid phase micro-extraction (SPME), which had a minimum detection limit of 5 µg/L. The data release shows that the abiotic degradation experiments showed no evidence...
These datasets contain microbial community data from groundwater samples collected at an in situ bioremediation site located at the former Naval Air Warfare Center (NAWC), West Trenton, NJ. DNA was extracted from groundwater samples collected from monitoring wells at the NAWC study site from July 2008 through July 2015 and analyzed for microbial community structure. Sample collection coincided with a groundwater bioremediation experiment investigating the microbial degradation of the contaminant trichloroethylene (TCE) prevalent in the targeted region of the aquifer. Nutrient addition and a microbial consortium, commercially developed to stimulate the degradation of TCE and TCE byproducts, was introduced to the...
Categories: Data;
Tags: 16S rRNA sequencing,
Environmental Health,
Genetics,
Naval Air Warfare Center,
Trichlorethylene,
A large spill of wastewater from oil and gas operations was discovered adjacent to Blacktail Creek near Williston, North Dakota in January 2015. To determine the effects of this spill on streambed microbial communities over time, bed sediment samples were taken from Blacktail Creek upstream, adjacent to, and at several locations downstream from the spill site. Blacktail Creek is a tributary of the Little Muddy River, and additional samples were taken upstream and downstream from the confluence of Blacktail Creek and the Little Muddy River. Samples were collected in February 2015, June 2015, June 2016, and June 2017. DNA was extracted from these sediments, and sequencing of the 16S ribosomal RNA gene was performed...
Categories: Data;
Tags: Aquatic Biology,
Blacktail Creek,
DNA sequencing,
Ecology,
Little Muddy River,
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