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Neonicotinoids have become the most widely used insecticides in world with rapid growth in applications as seed coatings. Nontarget organisms are exposed to concentrated levels of pesticidal active ingredients through ingestion of treated seeds. To better understand pesticide fate, analytical methods are necessary to rapidly screen and accurately quantitate contaminants in environmental and biological matrices. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly employed for neonicotinoid analyses but requires expensive analytical instrumentation and potentially laborious sample preparation. Enzyme-linked immunosorbent assays (ELISAs) are efficient and sensitive alternative methods for neonicotinoid...
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Zooplankton samples were collected at one site upstream of the Yolo Bypass in Northern California, three sites within the Bypass, and at a comparison site on the Sacramento River below the city of Sacramento and analyzed for a large suite of current-use pesticides and degradates. Samples were collected every two weeks from early August 2021 to mid-October 2021. Samples were collected by towing a 150-micron conical plankton net for 5 minutes by boat at each site. In the laboratory, zooplankton samples were filtered through a 63-micrometer sieve to separate the zooplankton and vegetation/detritus from the water. Large sticks, twigs, rocks, and leaves were rinsed with organic free water into the sieve and then discarded....
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Analysis of biological tissues or direct sources of food may better characterize exposure of non-target organisms to current-use pesticides. Food boluses were collected from tree swallow (Tachycineta bicolor) nestlings using a non-lethal, ligature method and were composited based on nest. The ligatures, which prevented the bolus from being swallowed, were placed on nestlings for 1 h until samples were collected. Samples in 2016 were collected from nestlings at 6 days and 12 days post-hatch whereas 2018 samples were collected from nestlings at 12 days post-hatch. Furthermore, in 2016 and 2018, insects commonly consumed by tree swallows were collected via sweep net and composited based on suborder (Brachycera, Nematocera,...
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Chemically intensive crop production depletes wildlife food resources, hinders animal development, health, survival, and reproduction, and it suppresses wildlife immune systems, facilitating emergence of infectious diseases with excessive mortality rates. Gut microbiota is crucial for wildlife’s response to environmental stressors. Its composition and functionality are sensitive to diet changes and environmental pollution associated with modern crop production. In the study entitled Exposure to crop production alters cecal prokaryotic microbiota, inflates virulome and resistome in wild prairie grouse we use shotgun metagenomics to demonstrate that exposure to modern crop production detrimentally affects cecal...
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Water primrose (Ludwigia hexapetala) is an invasive aquatic plant that has rapidly increased in coverage throughout the Sacramento-San Joaquin Delta. Water primrose has invaded wetlands and may contribute towards mortality of tules (Schoenoplectus spp.) and cattails (Typha spp.). Little research has been completed on the mechanisms responsible for marsh loss, but previous studies have suggested that water primrose contains allelopathic chemicals that could cause plant mortality. Three major allelopathic polyphenols (myricitrin, prunin, and quercitrin) previously identified in L. hexapetala leaves were measured in leaf, water, and soil samples from Delta marshes infested with water primrose. Samples were collected...
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In the United States and globally, the lack of data on contaminant exposure from unregulated private-well point-of-use (POU) tapwater (TW) is a recognized public health data gap and an obstacle to risk management and homeowner decision making. Mead, Nebraska, is an agricultural community about 30 miles west of Omaha. To help address the lack of data on broad contaminant exposures in TW sourced from private water wells, a total of 22 samples were collected from 18 private wells during June 2022 and January 2023 and analyzed for 495 organic compounds, 34 inorganic constituents, and 11 microbial groups. The organic compounds included pesticides, pharmaceuticals, volatile organic compounds (VOCs), disinfection byproducts...
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Japanese quail (Coturnix japonica) were orally dosed with pesticide-treated or control-untreated wheat seeds. Pesticide treated wheat seeds were analytically verified for active ingredients of imidacloprid, metalaxyl, tebuconazole, and fludioxonil. Pesticide and metabolite residues were measured in plasma, liver, brain, kidney, muscle, and excreta of exposed and control birds. Samples were extracted via solid phase extraction, pressurized liquid extraction, or sonication. Clean-up steps included protein precipitation followed by solid phase extraction. All samples were analyzed by liquid chromatography tandem mass spectrometry.
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- Observations of test subjects, - Body weight, organ/tissue weights - Biomarker data (oxidative DNA damage, thyroid hormones, corticosterone, gene expression) in various tissues - Residues as percent of administered dose - Tissues to plasma rations - Metabolites and ratios - Elimination half-lives


    map background search result map search result map Toxicokinetics of imidacloprid-coated wheat seeds in Japanese quail (Coturnix japonica) and evaluation of hazard Current-use pesticides in tree swallow (Tachycineta bicolor) prey Evaluation of ELISA for the Analysis of Imidacloprid in Plasma, Liver, and Fecal Matter Characterizing microbiota, virulome, and resistome of wild prairie grouse in crop producing and uncultivated areas of Nebraska Pesticide Concentrations Measured in Zooplankton Samples Collected from the Sacramento River and Yolo Bypass, California, 2021 Chemical Concentrations and Microbiological Results for Assessment of Mixed-Organic/Inorganic Chemical Exposures in Tapwater in Mead, Nebraska, June 2022 and January 2023 Analysis of Polyphenols in Water Primrose (Ludwigia hexapetala) Plants from the Sacramento-San Joaquin Delta of California Toxicokinetics of imidacloprid-coated wheat seeds in Japanese quail (Coturnix japonica) and evaluation of hazard Pesticide Concentrations Measured in Zooplankton Samples Collected from the Sacramento River and Yolo Bypass, California, 2021 Analysis of Polyphenols in Water Primrose (Ludwigia hexapetala) Plants from the Sacramento-San Joaquin Delta of California Chemical Concentrations and Microbiological Results for Assessment of Mixed-Organic/Inorganic Chemical Exposures in Tapwater in Mead, Nebraska, June 2022 and January 2023 Characterizing microbiota, virulome, and resistome of wild prairie grouse in crop producing and uncultivated areas of Nebraska Current-use pesticides in tree swallow (Tachycineta bicolor) prey Evaluation of ELISA for the Analysis of Imidacloprid in Plasma, Liver, and Fecal Matter