Filters: Tags: Bioassay (X) > partyWithName: Columbia Environmental Research Center (X)

Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Types Map Service (1) OGC WFS Layer (1) OGC WMS Layer (1) OGC WMS Service (1) Contacts Ann L Allert U.S. Geological Survey Jennifer C Brennan U.S. Geological Survey - ScienceBase CERC Data Managers More... Categories Data (1) Tag Types Theme (2) Place (2) Harvest Set (2) USGS Scientific Topic Keyword (1) Tag Schemes None (2) National Agricultural Library Thesaurus (1) USGS Thesaurus (1) USGS Biocomplexity Thesaurus (1) Common geographic areas (1) ISO 19115 Topic Category (1) View Results as: JSON ATOM CSV

Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Environmental Research Center, Missouri, USGS Science Data Catalog (SDC), bioassay, cells, All tags... fluorescence, luciferase, Fewer tags Field and laboratory data to determine lethal pesticide concentrations for control of invasive crayfish Data were collected associated with the application of a pesticide to a stormwater retention pond and burrows to suppress or eradicate an invasive crayfish species, Procambarus clarkii, in support of high-priority research developing control methods to mitigate impacts of invasive crayfish within the Great Lakes Basin. Effectiveness of the treatment was accessed using an in-situ bioassay and by measuring pesticide concentrations in water, sediment, and caged crayfish. Water quality data, including temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, and turbidity, in stormwater ponds was collected to evaluate whether environmental conditions may impact treatment effectiveness and... Tags: Aquatic Biology, Auburn Unversity, Columbia Environmental Research Center, Faxonius virilis, Procambarus clarkii, All tags... USGS Science Data Catalog (SDC), Upper Midwest Environmental Science Center, Water Quality, acute toxicity, aquatic biology, best management practices, bioassay, bioretention pond, burrows, chemical control, cypermethrin, ecosystem monitoring, environment, in-situ bioassay, invasive crayfish, invasive species, macroinvertebrates, pesticides, pyrethrin, red swamp crayfish, survival, total suspended solids, toxicity, toxicity testing, virile crayfish, water chemistry, water properties, water quality, Fewer tags