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This data archive includes R code and data for reproducing the analyses and figures in Lafferty, Metabarcoding is (usually) more cost effective than seining or qPCR for detecting tidewater gobies and other estuarine fishes. To view the supplementary tables, open the Fig&TableSuppl.docx file. This file also includes the manuscript figures and tables and some explanatory text about how to generate them. To reproduce the figures, open the Fig&TableCode.Rmd in R studio and be sure the needed csv files included in the Dryad repository are in the working directory. The data files include more information than used in the analyses and can be used for other purposes. The code is not software, nor is it intended as an...
This data set is comprised of one table with sampling information and National Center for Biotechnology Information (NCBI) BioProject accession numbers for sequence information of this amplicon-based study targeting Elodea canadensis and E. nuttallii in freshwater systems of Alaska from environmental samples. Highly conserved primers which can differentiate these species of interest were developed for four portions of Elodea mtDNA genes (ITS1-5.8S, atpB-rbcL, and two variations of trnL-trnF). The reference sequences and conserved primer sets to identify species present were developed using publicly available data from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/).
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