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  4. Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii Using Environmental DNA (eDNA), Alaska

Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii Using Environmental DNA (eDNA), Alaska

Dates

Publication Date
Start Date
2015
End Date
2018

Citation

Menning, D.M., 2024, Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii using environmental DNA (eDNA), Alaska: U.S. Geological Survey data release, https://doi.org/10.5066/P94ZAUGK.

Summary

This data set is comprised of one table with sampling information and National Center for Biotechnology Information (NCBI) BioProject accession numbers for sequence information of this amplicon-based study targeting Elodea canadensis and E. nuttallii in freshwater systems of Alaska from environmental samples. Highly conserved primers which can differentiate these species of interest were developed for four portions of Elodea mtDNA genes (ITS1-5.8S, atpB-rbcL, and two variations of trnL-trnF). The reference sequences and conserved primer sets to identify species present were developed using publicly available data from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/).

Contacts

Point of Contact :
Alaska Science Center
Originator :
Damian M Menning
Metadata Contact :
Alaska Science Center
Publisher :
Alaska Science Center
SDC Data Owner :
Alaska Science Center
USGS Mission Area :
Ecosystems
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

Click on title to download individual files attached to this item.

elodea_eDNA_Alaska_metadata.xml
Original FGDC Metadata
View 		42.42 KB application/fgdc+xml
elodeaAIS_eDNA_Alaska.csv 130.66 KB text/csv
elodea_eDNA_Alaska_metadata.html 80.2 KB text/html

Purpose

These data were collected to test the feasibility of using environmental DNA (eDNA) samples to detect the aquatic invasive species Elodea in Alaska.

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  • Alaska Science Center (ASC)

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Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P94ZAUGK

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