Filters: Tags: parasites (X) > partyWithName: National Wildlife Health Center (X)

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Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2... Categories: Data; Tags: Hawaii, USGS Science Data Catalog (SDC), Wildlife Disease, climatologyMeteorologyAtmosphere, parasites, All tags... pathology, spirorchiid, Fewer tags Carcass weights, 28S rRNA alignment file and parasite sample vouchers collected from California tiger salamanders (Ambystoma californiense) CTS and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) SCLT from Prospect or Ellicott Pond, on Ellicott Slough National Wildlife Refuge, California U.S.A. recorded September 11, 2019 Animal identification number, weight in grams for carcasses of California tiger salamanders (Ambystoma californiense) CTS and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) SCLT from Prospect or Ellicott Pond, on Ellicott Slough National Wildlife Refuge, California U.S.A. submitted to U.S. Geological Survey, National Wildlife Health Center (NWHC) for determination of cause of death. NWHC Case 29381. MSF Sequence file used to construct a phylogenetic tree. Sequences were generated from samples at NWHC (Px) and using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) they were compared for base pair similarities with publicly available sequences from GenBank. Sequences selected for the Multi Sequence... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: California tiger salamanders, Ellicott Slough National Wildlife Refuge, Ribeiroia ondatrae, Santa Cruz long-toed salamander, U.S. Geological Survey, National Wildlife Health Center, Madison, Wisconsin, All tags... USGS Science Data Catalog (SDC), Wildlife Disease, biota, parasites, Fewer tags Pathogenic lineage of Perkinsea causes mass mortality of frogs across the USA: Data (i) Severe perkinsea data was based on the observation of characteristic gross and microscopic lesions and the morphological identification of Perkinsea organisms within the lesions. Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (ii) Epidemiological information was compiled from each anuran sample, including the detection type (mortality event or health monitoring study), location (state and county), anuran species reported in the event, life-stage of species reported in the... Categories: Data; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: USGS Science Data Catalog (SDC), United States, amphibians, disease vectors, health and disease, All tags... parasites, wildlife mortality, Fewer tags LAMP assay for detection of Asian fish tapeworm Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls. Categories: Data; Tags: Asian fish tapeworm, LAMP assay, USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... eDNA, environmental DNA, invasive species, isothermal amplification, loop-mediated isothermal amplification, molecular detection, parasites, rapid assay, Fewer tags Parasite sample voucher numbers and 28S rRNA alignment file for parasites collected from California Giant Salamander (Dicamptodon ensatus) from Webb Creek, in the Bear Creek Redwoods Preserve in Santa Clara County, California, USA Multi sequence file (.msf) file used to construct a 28S rRNA phylogenetic tree of species of Euryhelmis and other heterophyid trematodes. Sequences were generated from metacercariae samples at U.S. Geological Survey National Wildlife Health Center (NWHC) and compared for base pair similarities with publicly available sequences from GenBank using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Selection of GenBank sequences to include in the .msf file was based on the number of base pair similarities (94% or higher identity to this study’s sequences), 98–100% coverage AND stage of parasite from which the sequence was generated. An excel file listing all parasites and DNA from NWHC vouchered with the University... Categories: Data; Tags: Bear Creek Redwoods Preserve, California Giant Salamander, Euryhelmis sp, Santa Clara, USGS Science Data Catalog (SDC), All tags... Webb Creek, Wildlife Disease, biota, infection, parasites, Fewer tags