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We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured...
These data include Ceratocystis culture viability results from ambrosia beetle (Coleopetera: Scolytinae) frass. Frass is defined as fine particles of macerated wood or boring dust, beetle parts, and feces. Frass was collected from individual ambrosia beetle galleries in Ceratocystis-infected ʻōhiʻa (Metrosideros polymorpha) trees on Hawaiʻi Island, a phenomenon referred to as Rapid ʻŌhiʻa Death (ROD). We recorded the height at which these beetles produced frass, how long we collected frass from galleries, and the total number of culturing tests from individual galleries.
These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of ʻōhiʻa trees, soil, and seedlings.
Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps...
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Management,
These data include the quantitative Polymerase Chain Reaction (qPCR) Ceratocystis lukuohia and C. huliohia DNA confirmations of viable subcultures. These data were used to determine the accuracy of morphological identifications.
Environmental DNA (eDNA) detection tools are becoming increasingly popular for documenting occurrence and distribution of native and invasive species. These tools can allow early detection of new diseases and invasive species and provide critical information for land management. We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). This dataset contains results of an experiment that was designed to determine probability of detecting known numbers of Ceratocystis lukuohia spores on individual slides in these samplers.
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Metrosideros polymorpha,
These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible...
We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results...
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Metrosideros polymorpha,
These data include frass production estimates from three ambrosia beetle species. Frass was collected from individual ambrosia beetle galleries in Rapid 'Ōhiʻa Death-infected ʻōhiʻa trees (Metrosideros polymorpha) from 2018-2019. Frass is defined as fine particles of macerated wood or boring dust, beetle parts, and feces.
This data release includes monthly census data of 120 out-planted ʻōhiʻa seedlings in different treatment groups and associated metadata from February 2019 - February 2020 at Keaukaha Military Reservation in Hilo, HI. Six seedlings each were planted around 20 focal trees, half of which tested positive for Rapid ʻŌhiʻa Death, half of which tested negative. Three of the six seedlings were weeded for nearby invasive species and the other three were not. Initial height of seedlings, whether they were damaged at time of planting, and whether they were initially grown in a dibble tube or pot was also recorded. After initial damage to many seedlings by ungulates, we decided to apply a fencing treatment for ungulate exclusion...
These data include the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts (tree stem sections) infected with Rapid ʻŌhiʻa Death on Hawaiʻi Island from 2018-2019. These data were used to determine species, number of individuals reared, height, and elevation in which these beetles live.
This data release includes data and metadata containing (1) study site locations and elevation where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island, (2) the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts, (3) Ceratocystis spp. viability results from culturing tests of the beetles' frass, (4) frass production estimates from three ambrosia beetle species, and (5) quantitative Polymerase Chain Reaction (qPCR) DNA results of viability subcultures. Data were used in the analysis for the manuscript "Ambrosia beetle communities and frass production in ʻōhiʻa (Metrosideros polymorpha) infected with Ceratocystis fungi responsible...
These data contain ambrosia beetle (Coleoptera: Curculionidae) trapping data using two different repellents in two different trials at Waiākea Forest Reserve. Beetles were trapped using cross vane panel traps equipped with 50:50 Ethanol:Methanol lures in the first trial and with black panel multi-traps equipped with 100% Ethanol lures in the second trial. Both trials tested the ability of two different beetle repellents, one verbenone formulation, and one verbnone + methyl salicylate, to deter beetle catch.
This data release includes data and metadata containing (1) ambrosia beetle (Coleoptera: Curculionidae) trapping data using two different lures at Waiākea Forest Reserve and ʻŌlaʻa Forest of Hawai'i Volcanoes National Park, (2) ambrosia beetle trapping data using paired lures and repellents from two trials conducted at Waiākea Forest Reserve, and (3) the localities of all traps used in both trapping experiments. Data were used in the manuscript titled, "The use of semiochemicals for attracting and repelling invasive ambrosia beetles (Coleoptera: Curculionidae) in Rapid ʻŌhiʻa Death-infected forests."
We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw particle sizes of polyethylene spheres that were...
We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust (frass) known to contain fungi responsible for Rapid ʽŌhiʽa Death. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust....
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental DNA,
Environmental Sampler,
Environmental Sampler,
Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Envrionmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers for collecting airborne particulates and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental...
Categories: Data;
Tags: Environmental Sampler,
Hawaii,
Management,
Metrosideros polymorpha,
Ohia lehua,
Data were collected to ascertain whether restoration is possible in ROD-affected areas. We addressed this question by testing soils and adult ʻōhiʻa trees for ROD-causing Ceratocystis DNA and viable spores before outplanting young ʻōhiʻa adjacent to ROD-positive and ROD-negative trees in these environments.
These data contain the global positioning system (gps) coordinates and elevation data of traps used to test different ambrosia beetle (Coleoptera: Curculionidae) lures and repellents in two different experiments conducted at Waiākea Forest Reserve and ʻŌlaʻa Forest of Hawai'i Volcanoes National Park.
This data release includes metadata and tabular datasets that document (1) Austropuccina, Ceratocystis and Myrtaceae qPCR (quantitative polymerase chain reaction) DNA detections in Passive Environmental Samplers (PES), (2) wind speed, wind gust speed, and wind direction measurements collected at two sites in the Kahuku Unit of Hawai'i Volcanoes National Park (HAVO) where paired PES were located, (3) localities, sites and elevations where PES were located, and (4) Genbank accession numbers for Austropuccinia and Ceratocystis DNA sequences amplified from samples collected in a subset of PES. These raw data were analyzed and reported in the manuscript "Environmental Monitoring for Invasive Fungal Pathogens of ʽŌhiʽa...
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