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Filters Date Range Past year (14) Past month (0) Past week (0) Past day (0) Custom range... To   Search Contacts Kylle A Roy Robert W Peck U.S. Geological Survey - ScienceBase Pacific Island Ecosystems Research Center U.S. Geological Survey More... Categories Data (22) Data Release - Revised (1) Tag Types Theme (173) Place (34) Tags (with Scheme=None) environmental dna (13) environmental sampler (10) hawaiii (9) invasive fish (9) spore traps (9) metrosideros polymorpha (8) ohia lehua (8) More... View Results as: JSON ATOM CSV

Polyethylene Particle Detection (counts) during Environmental Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured... Categories: Data; Tags: Environmental DNA, Environmental Sampler, Hawaiii, Spore Traps, environment Hawaii Island, carbon dioxide as a tool to manage invasive fish in anchialine pools, initial aquarium water conditions, 2020 This data release includes metadata and tabular data that documents initial water conditions (temperature, pH, dissolved oxygen and salinity) during aquarium trials testing the efficacy of carbon dioxide (CO2) to manage invasive fish in anchialine pools. Categories: Data; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, anchialine pool, All tags... biota, carbon dioxide, invasive fish, invasive species management, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, primer specificity, 2019-2022 This data release documents specificity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022. Categories: Data; Tags: Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, biota, environmental DNA, All tags... invasive fish, quantitative PCR, Fewer tags Orchidlands Estates Ceratocystis DNA Detection 2016 Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps... Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Management, All tags... Metrosideros polymorpha, Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Hawaii Island, carbon dioxide as a tool to manage invasive fish in anchialine pools, aquarium trials subject fate, 2020 This data release includes metadata and tabular data that documents the survival of fish or invertebrate exposed to carbon dioxide (CO2) gas treatment during aquarium trials. Aquarium trials were conducted with six taxa: guppies (Poecilia reticulata), western mosquitofish (Gambusia affinis), Mozambique tilapia (Oreochromis mossambicus), Opae ula shrimp (Halocaridina rubra), feeble shrimp (Palaemon debilis) and a species of thiarid snail (Thiaridae). These results were part of a larger study aimed to test the efficacy of CO2 as a tool for managing invasive fish in anchialine pools. Categories: Data; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, anchialine pool, All tags... biota, carbon dioxide, invasive fish, invasive species management, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide as a tool to manage invasive fish in anchialine pools, field pH levels during treatment, 2021-2022 This data release includes metadata and tabular data that documents changes in pH of anchialine pool water before, during and after diffusion of carbon dioxide (CO2) gas. These results are part of a study testing the efficacy of CO2 as tool for eradicating invasive fish from anchialine pools. The trials were conducted at Puuhonua o Honaunau National Historical Park and Kaloko-Honokohau National Historical Park, Hawaii, during 2021-2022. Categories: Data; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, anchialine pool, All tags... biota, carbon dioxide, invasive fish, invasive species management, Fewer tags Effect of SDS and Proteinase K on whole blood preserved in lysis buffer for Hawaiian Island avian malaria detection, 2005-2006 It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic... Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, enzyme linked immunosorbant assay, farming, immunoblot, polymerase chain reaction, Fewer tags Ceratocystis lukuohia spore dilution for probit analysis Environmental DNA (eDNA) detection tools are becoming increasingly popular for documenting occurrence and distribution of native and invasive species. These tools can allow early detection of new diseases and invasive species and provide critical information for land management. We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). This dataset contains results of an experiment that was designed to determine probability of detecting known numbers of Ceratocystis lukuohia spores on individual slides in these samplers. Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Metrosideros polymorpha, All tags... Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Hawaii Island Environmental Sampler Comparison 2016-2018 These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible... Tags: Ceratocystis, Ecology, Environmental DNA, Environmental Health, Environmental Sampler, All tags... Forestry, Genetics, Hawaiii, Metrosideros polymorpha, Ohia lehua, Rapid Ohia Death, Spore Traps, USGS Science Data Catalog (SDC), Xyleborus, environment, Fewer tags Ceratocystis DNA Detection in Frass During Caged Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results... Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Metrosideros polymorpha, All tags... Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Derived ELISA Values for Hawaiian Island forest bird avian malaria detection using whole blood preserved in lysis buffer It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm. Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags Hawaii Island, carbon dioxide as a tool to manage invasive fish in anchialine pools, aquarium treatment observations, 2020 This data release includes metadata and tabular data that documents the behavioral response of three species of fish invasive to Hawaii ( tilapia [Oreochromis mosambicus], western mosquitofish [Gambusia affinis] and guppies [Poecilia reticulata]) during aquarium trials testing the efficacy of carbon dioxide (CO2) diffused in water to manage invasive fish in anchialine pools. The work was conducted during 2020. Categories: Data; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, anchialine pool, All tags... biota, carbon dioxide, invasive fish, invasive species management, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023) This data release includes metadata and tabular data that documents lab and field trials testing the efficacy of carbon dioxide (CO2) gas diffused into water to manage invasive fish in anchialine pools. The data release also includes information documenting sensitivity, specificity, and accuracy of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia (Oreochromis mosambicus), western mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata). In total there are 11 datasets, 9 describing lab trials and 2 describing field trials. Lab data include 1) initial water conditions during pilot study, 2) behavioral response of fish to CO2 during pilot study, 3) survival... Categories: Data, Data Release - Revised; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, USGS Science Data Catalog (SDC), All tags... anchialine pool, biota, carbon dioxide, eDNA, invasive fish, invasive species management, qPCR assays, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, evaluation of assays, 2019-2022 This data release documents results of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies in pools with known fish populations and in pre and post-treatment samples from pools that were treated with carbon dioxide gas. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022. Categories: Data; Tags: Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, biota, environmental DNA, All tags... invasive fish, quantitative PCR, Fewer tags Waipunalei ROD Management 2017-2018 This data release includes metadata and tabular datasets that document (1) Ceratocystis qPCR DNA detections in Passive Environmental Samplers (PES), (2) Ceratocystis qPCR DNA detections after pre-amplification, (3) wood and frass particle counts detected in PES (4) location of individual PES within the study area (5) ambrosia beetle gallery counts on felled ʻōhiʻa (6) ambrosia beetle gallery counts on insecticide treated bolts and (7) ambrosia beetles trapped in cross-vein panel traps. Data were used in the analysis of the manuscript "Effectiveness of Rapid ʻŌhiʻa Death management strategies at a focal disease outbreak on Hawaiʻi Island." Tags: Beetle frass, Ceratocystis huliohia, Ceratocystis lukuohia, Ecology, Hawaii Island, All tags... Hawaiii, Management, Ohia lehua, Passive Environmental Samplers, Rapid Ohia Death, USGS Science Data Catalog (SDC), environment, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, limit of detection, 2019-2022 This data release documents sensitivity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022. Categories: Data; Tags: Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, biota, environmental DNA, All tags... invasive fish, quantitative PCR, Fewer tags Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006 It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered... Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide as a tool to manage invasive fish in anchialine pools, fish body length measurements, 2021-2022 This data release includes metadata and tabular data that documents the total body length of Mozambique tilapia (Oreochromis mossambicus) removed from anchialine pools during field trials testing the efficacy of carbon dioxide (CO2) gas as tool for eradicating invasive fish. Tilapia were removed from a pool at Puuhonua o Honaunau National Historical Park, Hawaii during 2021-2022. Categories: Data; Tags: Hawaii, Hawaii Island, Koloko-Honokohau National Historical Park, Puuhonua o Honaunau National Historical Park, anchialine pool, All tags... biota, carbon dioxide, invasive fish, invasive species management, Fewer tags Hawaii Island, modelled density of malaria-resistant and -susceptible Iiwi following release of malaria-resistant birds under three climate change projections, 2030-2100 This data set provides the simulated results of releasing malaria-resistant Iiwi into existing populations of wild birds on the Island of Hawaii. Resistant birds are released into mid- and high-elevation forests at different densities at 10-year intervals from 2030 to 2070. Populations of both malaria-resistant and susceptible Iiwi are then predicted at 10-year intervals from release until 2100. Predictions are made based on 3 different climate change projections: A1B, RCP4.5, and RCP8.5. The goal of this project is to evaluate the feasibility of creating a successful population of Iiwi when faced with higher malaria infection predicted as a result of climate change. The model results presented here build upon two... Categories: Data; Tags: CRISPR, Ecology, Hawaiian forest birds, Iiwi, Island of Hawaii, All tags... USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, avian malaria, biota, climate change, facilitated adaptation, gene editing, Fewer tags Sphere Particle Measurements During Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw particle sizes of polyethylene spheres that were... Categories: Data; Tags: Environmental DNA, Environmental Sampler, Hawaiii, Spore Traps, environment 1 2 »