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Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
Alternative complement pathway assay data observed in adult largemouth bass plasma in response to an EE2 (17alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions, with associated fish identification information.
Baseline fish health information on pallid sturgeon and bull trout or appropriate surrogate species (shovelnose sturgeon, cutthroat trout, mountain whitefish) in critical habitats with elevated risks of oil spills. Shovelnose sturgeon (Scaphirhynchus platorynchus) were collected in three locations near the confluence of the Missouri and Yellowstone Rivers and cutthroat trout (Oncorhynchus clarki lewisi) and mountain whitefish (Prosopium williamsoni) were collected from critical bull trout habitat along the Middle Fork of the Flathead River. Fish health metrics evaluated included gene expression/enzyme induction in various tissues, differential changes in white blood cells, polycyclic aromatic hydrocarbon bile metabolites,...
Monitoring of vitamin B1 (thiamine) in the eggs of lake trout (Salvelinus namaycush) collected from lakes Huron and Michigan has been conducted by the U.S. Geological Survey since the mid-1990’s. The results of this monitoring effort have been used by natural resource management agencies and native American tribes within the Great Lakes basin to evaluate the reproductive health of lake trout. The data in this release are the thiamine concentrations measured in lake trout collected from 1996-2018. Results are reported as total thiamine (nmol/g-egg), collection site, lake, year collected, and reference to the analysis methods.
Categories: Data;
Tags: Canada,
EMS,
Early Mortality Syndrome,
Fish,
Fish Spawning and Larval Aggregation,
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17α-ethinylestradiol.
In crude-oil-contaminant plumes the dissolved organic carbon (DOC) is mainly hydrocarbon degradation intermediates only partly quantified by the diesel range total petroleum hydrocarbon (TPHd) method. To understand potential biological effects of degradation intermediates we tested three fractions of DOC: (1) solid phase extract (HLB); (2) dichloromethane (DCM-total) extract used in TPHd; and (3) DCM extract with hydrocarbons isolated by silica gel cleanup (DCM-SGC). Bioactivity of extracts from five wells spanning a range of DOC was tested using an in vitro multiplex reporter system that evaluates modulation of activity of 46 transcription factors; extracts were evaluated at concentrations equivalent to the well...
This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates...
Groundwater samples were collected in June 2018 from a background (reference) well located 200 m upgradient from the source and five wells along a flowline in the plume at 39, 68, 102, 125, and 254 m downgradient from the source. Before sampling, at least three times the water volume in the well casing was purged and field parameters (temperature, dissolved oxygen, specific conductance, and pH) were stable. Two samples from each well were collected into unpreserved 1 L amber bottles and shipped on ice overnight to a commercial lab. The two samples were extracted using dichloromethane (DCM; EPA Method 3510). One sample extract was treated with silica gel cleanup (SGC) column (USEPA method 3630C). Aliquots of the...
This data describes the relative luminescence units (RLUs) and relative fluorescence units (RFUs) of lysates of aryl-hydrocarbon responsive chemically-activated luciferase expression human hepatoma HG2L7.5c1 cells which were exposed for 4 or 24 h to beta-naphthoflavone dilutions, 2,3,7,8-tetrachlorodibenzo-p-dioxin dilutions, or dilutions of groundwater extracts collected from wells within or near an oil-contaminated area. This data also describes RFUs of bovine serum albumin standards which were used to convert the RFUs of the cell lysates to protein with which to normalize the RLUs of the cell lysates.
Acute toxicity and clotting times of anticoagulant rodenticides to four fish species.
Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter), or a mixture of endocrine-active substances commonly detected in the Chesapeake Bay watershed, atrazine (5.4 micrograms per liter) and estrone (47.9 nanograms per liter). Male reproductive condition, sperm count, and sperm motility were assessed.
Early rearing of steelhead (Oncorhynchus mykiss) in Oregon hatcheries is often problematic; fry can become emaciated and die during the period between hatch and first feed. Thiamine (vitamin B1) deficiency has caused early mortality in salmonids; however, thiamine status of Oregon’s anadromous steelhead populations is currently unknown. We sampled eggs of 7-10 females during spawning from three Oregon hatcheries in 2019. In 2022, females were injected with buffered thiamine HCl 50 mg/kg prior to spawning; additionally, a subset of eggs were supplemented via bath treatment with thiamine mononitrate (1000 ppm) at spawning. We then compared egg thiamine from control and injected females, as well as measured mortality...
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