Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects

Lake Sinai Viruses (LSV) are common ribonucleic acid (RNA) viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. We developed a new genetic assay that detects all currently known lineages. We also performed pilot metagenetic sequencing to quantify the diversity of LSV...

Tags: California, Genetics, Insects, Maryland, Nebraska, All tags... North Dakota, USGS Science Data Catalog (SDC), Viruses, Wildlife Disease, biota, farming, Fewer tags

Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters

The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read sequencing (Illumina MiSeq and Ion Torrent Personal Genome Machine) of long-range PCR products amplified from tumor tissue using primers designed from the single available ChHV5 reference genome from a Hawaii green sea turtle. The raw sequence data is available at the NCBI Sequence Read Archive under bioproject number PRJNA360405, including...

Tags: Class Reptilia, DNA sequencing, Earth, Oceans, Atlantic, North Atlantic, Earth, Oceans, Pacific, Family Cheloniidae, All tags... Family Herpesviridae, Florida, Indian River County, Martin County, Sarasota, Genetic Diversity, Genetics, Genus Chelonia, Genus Scutavirus, Green Sea Turtle, Hawaii, Hilo, Hawaii, Maui County 2, Hawaii, Oahu, Honolulu County, Infrakingdom Deuterostomia, Infraphylum Gnathostomata, Kingdom Animalia, Order Herpesvirales, Order Testudines, Phylum Chordata, Species Chelonia mydas, Species Chelonid alphaherpesvirus 5, Subfamily Alpheherpesvirinae, Subfamily Cheloniinae, Subkingdom Bilateria, Suborder Cryptodira, Subphylum Vertebrata, Superclass Tetrapoda, Superfamily Chelonioidea, USGS Science Data Catalog (SDC), Virology, Wildlife Disease, biota, disease research, endangered species, genetics, marine biology, polymerase chain reaction, reptiles, sea turtles, virology, viruses, Fewer tags

Metagenomic detection and reconstruction of Lake Sinai Virus from honey bee sequence data

A survey of public honey bee sequence data was performed to detect infections by Lake Sinai Virus (LSV). The Sequence Read Archive of the National Center for Biotechnology Information (NCBI) was queried to identify accessions of RNA sequence data derived from honey bee. These were filtered as described below and then up to 50 million reads or read pairs were downloaded and searched against a reference database of conserved LSV sequence. Accessions with matches above a specified threshold were downloaded in their entirety and assembled into longer contiguous sequences (contigs). The result contigs were searched against each open reading frame (ORF) of the reference LSV genome present in the NCBI database (accession...

Tags: RNA, USGS Science Data Catalog (SDC), Viruses, climatologyMeteorologyAtmosphere, farming