Filters: Tags: avian malaria (X) > partyWithName: Carter T Atkinson (X)

Filters Date Range Past year (2) Past month (0) Past week (0) Past day (0) Custom range... To   Search Contacts U.S. Geological Survey - ScienceBase Hawaii Cooperative Studies Unit Pacific Island Ecosystems Research Center Dennis Lapointe Michael D Samuel Ecosystems U.S. Geological Survey Categories Data (5) Tag Types Theme (6) Place (6) USGS Scientific Topic Keyword (1) Harvest Set (2) Tag Schemes None (6) ISO 19115 Topic Category (6) View Results as: JSON ATOM CSV

Effect of SDS and Proteinase K on whole blood preserved in lysis buffer for Hawaiian Island avian malaria detection, 2005-2006 It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic... Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, enzyme linked immunosorbant assay, farming, immunoblot, polymerase chain reaction, Fewer tags Derived ELISA Values for Hawaiian Island forest bird avian malaria detection using whole blood preserved in lysis buffer It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm. Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006 It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered... Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags Hawaii Island, modelled density of malaria-resistant and -susceptible Iiwi following release of malaria-resistant birds under three climate change projections, 2030-2100 This data set provides the simulated results of releasing malaria-resistant Iiwi into existing populations of wild birds on the Island of Hawaii. Resistant birds are released into mid- and high-elevation forests at different densities at 10-year intervals from 2030 to 2070. Populations of both malaria-resistant and susceptible Iiwi are then predicted at 10-year intervals from release until 2100. Predictions are made based on 3 different climate change projections: A1B, RCP4.5, and RCP8.5. The goal of this project is to evaluate the feasibility of creating a successful population of Iiwi when faced with higher malaria infection predicted as a result of climate change. The model results presented here build upon two... Categories: Data; Tags: CRISPR, Ecology, Hawaiian forest birds, Iiwi, Island of Hawaii, All tags... USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, avian malaria, biota, climate change, facilitated adaptation, gene editing, Fewer tags Hawaii Island forest bird avian malaria detection using whole blood preserved in lysis buffer, 2005-2006 It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with... Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, USGS Science Data Catalog (SDC), Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags Raw absorbance values for serially diluted whole blood preserved in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006 It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from infected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline... Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags