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This data release documents specificity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
This data was collected by the US Fish and Wildlife Service to see if environmental DNA (eDNA) varied across pools and within pools in the Illinois River basin. The data was collected in 2015 from three different habitat types: shoreline, main channel, and bays. The resulting data were then analyzed using an occupancy model.
Categories: Data;
Tags: Illinois,
USGS Science Data Catalog (SDC),
biota,
environment,
environmental DNA,
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the following streams: Big Creek, Mill Creek, Prewitt Creek, and Willow Creek. The metadata also includes the distance (meters) of each eDNA sample site from the stream mouth, volume of water (Liters) collected for eDNA analysis at each site, and the Y-intercept, slope, and R-squared value for each assay run.
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
Categories: Data;
Tags: Columbia Environmental Research Center,
climatologyMeteorologyAtmosphere,
eDNA,
eDNA Shedding rates,
environmental DNA,
Water samples from the Platte River were collected and analyzed for environmental DNA (eDNA). The U.S. Geological Survey (USGS) Nebraska Water Science Center (NEWSC) collected samples monthly from November 2019 to September 2022 at the Platte River near Ashland, Nebraska (USGS: 06801000). The USGS, in cooperation with the City of Lincoln, collected these samples. Water from the Platte River was collected as a series of weighted bottle subsamples that were taken from equal width increments across the river and composited into a sterilized 1-liter bottle. An aliquot of this composited sample was extracted using a sterile syringe and then filtered by the USGS NEWSC staff using filters provided by Jonah Ventures. These...
This data set is comprised of one table with sampling information and NCBI BioProject accession numbers for sequence information of this amplicon-based study targeting Labyrinthula, Phytophthora, and Halophytophthora of known pathogenic and non-pathogenic [to eelgrass (Zostera marina)] strains from eDNA samples. eDNA samples included water, sediment, and eelgrass from Notsuke Wan (Cove) Japan, Safety Sound, Izembek Lagoon, Port Moller, Chignik Lagoon, and Frederick Sound, Alaska and cloacal swabs from waterfowl hunted near Cold Bay Alaska. Replicate samples and multiple sampling dates of the same location were included. Highly conserved primers which could differentiate species of interest were developed for four...
Categories: Data;
Tags: Alaska,
Animals/Vertebrates,
Aquatic Biology,
Aquatic ecosystems,
Biological informatics,
These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible...
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for mucket (Actinonaias ligamentina) and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing food avilability scenarios. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
Categories: Data;
Tags: Columbia Environmental Research Center,
climatologyMeteorologyAtmosphere,
eDNA,
eDNA Shedding rates,
environmental DNA,
This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).
This data release documents results of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies in pools with known fish populations and in pre and post-treatment samples from pools that were treated with carbon dioxide gas. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
The dataset is composed of four tables containing data collected during screening surveys for invasive Round Goby (Neogobius melanostomus) at 12 sites on the Eastern Erie Canal in New York between Oneida Lake and the Hudson River from 2016 to 2023. The sampling techniques and frequency of sampling were adapted over time to optimize the efficiency of the study effort. From 2016-2019 eDNA water sampling and traditional fish surveys using seining, trapping, and benthic trawling were conducted twice annually (spring and summer) at the 12 sites. From 2020-2022, eDNA water sampling and traditional fish surveys using benthic trawling and electrofishing were conducted twice annually (spring and summer). In 2023, benthic...
Categories: Data,
Data Release - Revised;
Tags: Ambient Monitoring,
Aquatic Biology,
Aquatic Community Health,
Eastern Erie Canal,
Ecosystem Health,
The dataset is composed of four tables containing environmental DNA (eDNA) data, fish capture data, and site location information from Round Goby (Neogobius melanostomus) surveys conducted on parts of the Champlain Canal and upper Hudson River, and adjacent areas, in New York during 2022, 2023, and 2024. First posted May 5, 2022, ver. 1.0 Revised June 2022, ver. 2.0 Revised August 2022, ver. 3.0 Revised November 2022, ver. 4.0 Revised February 2023, ver. 5.0 Revised May 2023, ver. 6.0 Revised June 2023, ver. 7.0 Revised September 2023, ver. 8.0 Revised October 2023, ver. 9.0 Revised December 2023, ver. 10.0 Revised June 2024, ver. 11.0 Revised August 2024, ver. 12.0
Data describe specificity and sensitivity testing for the L.sil2 assay. This assay is used to amplify eDNA from the freshwater mussel Lampsilis siliquoidea.
Categories: Data;
Tags: Aquatic Biology,
Boone County, Missouri,
Columbia Environmental Research Center,
USGS Science Data Catalog (SDC),
biota,
This data release documents sensitivity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.
Abstract: Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: EARTH SCIENCE > LAND SURFACE > LANDSCAPE,
Federal resource managers,
LCC Network Science Catalog,
Report,
State agencies,
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.
Categories: Data,
Data Release - Revised;
Tags: Big Piney River, Missouri,
USGS Science Data Catalog (SDC),
biota,
environment,
environmental DNA,
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska and analyzed with quantitative PCR.
Categories: Data;
Tags: Alaska,
Aquatic Biology,
USGS Science Data Catalog (SDC),
biota,
environmental DNA
This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published primer sets designed to amplify freshwater mussels, one that amplified the mitochondrial cytochrome c oxidase I (COI) region and one that amplified the NADH dehydrogenase subunit I region, with negative control and mock community samples included. Samples were individually indexed, pooled, and sequenced on an Illumina MiSeq high-throughput...
This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina and Cumberlandia monodonta from water samples (eDNA).
Categories: Data;
Tags: Boone County, Missouri,
Columbia Environmental Research Center,
Genetics,
USGS Science Data Catalog (SDC),
biota,
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