Filters: Tags: fungi (X) > partyWithName: Great Lakes Science Center (X)
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The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
Description of Work The invasive form of Phragmites australis (common reed) is a well-established pest in many parts of the Great Lakes and the Atlantic, Gulf, and Pacific coasts, including designated Areas of Concern. New innovative control options that sustainably target the competitive advantage often enjoyed by Phragmites and other invasive plants will contribute to a broad Integrated Pest Management (IPM) strategy. This project targets the microorganisms that may help Phragmites spread and will employ a molecular genetic approach to silence the genes in Phragmites that give it a competitive edge over many native plants. This project helped build and will continue to be closely aligned with the Great Lakes Phragmites...
Categories: Project;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Areas of concern,
Ecosystem health,
GLRI,
Governmental,
Great Lakes Restoration Initiative,
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