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  4. Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli

Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli

Dates

Publication Date
Start Date
2012-12-01
End Date
2013-02-28

Citation

Richter, C.A., 2022, Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli: U.S. Geological Survey data release, https://doi.org/10.5066/P9DGI5F5.

Summary

Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2012 and 2013.

Contacts

Point of Contact :
Cathy A Richter
Originator :
Cathy A Richter
Metadata Contact :
CERC Data Managers
Publisher :
U.S. Geological Survey
Data Owner :
Columbia Environmental Research Center
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Columbia Environmental Research Center
USGS Mission Area :
Ecosystems

Attached Files

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Polyacrylamide_gel_electrophoresis.xml
Original FGDC Metadata
View 		38.86 KB application/fgdc+xml
Polyacrylamide_gel_electrophoresis.txt 5.35 KB text/plain
Gel_Diagram.docx 256.75 KB application/vnd.openxmlformats-officedocument.wordprocessingml.document

Purpose

Polyacrylamide gel electrophoresis experiments with thiaminase activity stain were conducted to test the function of genes encoding putative thiaminases from several species of fish.

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Communities

  • Columbia Environmental Research Center (CERC)
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9DGI5F5

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