Filters: partyWithName: U.S. Geological Survey - ScienceBase (X) > partyWithName: Carl O Ostberg (X)
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The data support a study that describes the development and validation of a primer and probe based quantitative PCR (qPCR) assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The metadata includes qPCR quantification cycle (Cq) values from testing the A. gracile assay on DNA extracted from tissue samples derived from several A. gracile and closely related species and Cq values from testing the assay on environmental DNA (eDNA ) samples collected from two lakes, one containing A. gracile and one without the species.
Categories: Data;
Tags: Aquatic Biology,
Genetics,
Pacific Northwest,
USGS Science Data Catalog (SDC),
amphibians,
The data support a study that surveyed the spatial and temporal distribution of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020. The metadata represent qPCR quantification cycle (Cq) values for Chinook salmon, coho salmon, and coastal cutthroat trout assays performed on water samples collected on specific days at specific sites on Thornton Creek, Taylor Creek, and Mapes Creek, which are tributaries of Lake Washington within Seattle city limits. The metadata also includes latitude and longitude for each site and Y-intercept and slope for each assay run.
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the following streams: Big Creek, Mill Creek, Prewitt Creek, and Willow Creek. The metadata also includes the distance (meters) of each eDNA sample site from the stream mouth, volume of water (Liters) collected for eDNA analysis at each site, and the Y-intercept, slope, and R-squared value for each assay run.
Introduction and translocation programs require thoughtful planning to increase the likelihood of success and to understand the benefits, risks, and constraints of such programs. A risk assessment was completed for bull trout introduction into the Sullivan Lake and Harvey Creek watershed, northeastern Washington. The risk assessment was designed to evaluate potential risks to resident fish species, to bull trout introduced into Sullivan Lake, and to bull trout donor source populations. The risk assessment describes the potential risks associated with pathogens, genetics, and ecological interactions. Literature reviews were completed for fish species composition and abundance in Sullivan Lake watershed to assess...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Aquatic Biology,
British Columbia,
Ecology,
Genetics,
Idaho,
We collected environmental DNA (eDNA) data from the Elwha River, home to the world’s largest dam removal project, to track the spatial and temporal patterns of species responses following dam removal. In total, we collected data for 11 different fish taxa, sampled at 25 sites ranging across 56 river kilometers in a wilderness river for 4 years following dam removal. We show that eDNA can effectively be used to determine whether fish have recolonized past former dams, and in some cases determine the spatial extent of that recolonization.
There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019 and 74 sites in spring 2020. Our surveillance identified zones with high waterborne parasite loads and provides a proof of concept for this approach that could be expanded throughout the larger Lake Washington watershed.
Categories: Data;
Tags: Aquatic Biology,
Ceratonova shasta,
Ecology,
Parvicapsula minibicornis,
Puget Sound,
We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes with varying species abundances, and a subset of lakes were re-sampled to test temporal variability in detection.
Categories: Data;
Tags: Aquatic Biology,
Downloadable Data,
Ecology,
Egeria densa,
Myriophyllym spicatum,
These data contain all the raw results needed to support the conclusions for the final product. These data are water sampling locations (latitude and longitude), date of water sampling, quantitative PCR values for each water sample, and stream flow at USGS stream gauging stations on sampling day.
This release includes data used to evaluate the structure and function of the Ross Lake and Diablo Lake food webs. This includes data on zooplankton density and production (zooplankton_density.csv and daphnia_region_production_biomass.csv), lake volume estimates used to expand zooplankton density and production data (lake_volume_estimates.csv), fish sampling (FishSampleEvents.csv), fish biological information including diets, age, and stable isotope analysis (FishFullData_formatted.csv, FishPreyLength.csv), scale back-calculations (salmonid_back_calc.csv, rss_back_calc.csv), fish energy density (calorimetry_processed.csv), stable isotope data for invertebrate end members (si_inverts.csv), and hydroacoustic sampling...
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Limnology,
Ross Lake National Recreation Area,
USGS Science Data Catalog (SDC),
Columns of data representing Chehalis River tributary, sample reach locations, dates of water sampling, quantitative PCR results, and whether lamprey were sampled by electrofishing in each sample reach. ***Please note that the title of this data release used to be "Environmental DNA surveys for lampreys in Chehalis River tributaries, Washington, from 2015 and 2017," but was updated for version 2.0.
Categories: Data,
Data Release - Revised;
Tags: Chehalis River,
USGS Science Data Catalog (SDC),
biota,
environmental DNA,
lampreys
We conducted a lab study to explore eDNA shedding during early life history (from fertilized eggs until near yolk sac absorption) in Chinook Salmon at three biomasses: 10 eggs, 100 eggs, and 1,000 eggs, and each egg biomass was replicated (indicated as replicates 1 and 2). Water samples were collected at several time points before, during, and after hatch for detection of Chinook Salmon DNA. On days when water samples were collected, we counted the number of dead eggs and the number of viable eggs hatched (identified as larvae emerged from eggs) until hatching was completed. The dataset represents the results from testing water samples for Chinook Salmon DNA using a quantitative polymerase chain reaction (qPCR)...
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