Filters: partyWithName: U.S. Geological Survey - ScienceBase (X) > partyWithName: Tonie E Rocke (X)

Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Types Map Service (5) OGC WFS Layer (5) OGC WMS Layer (5) OGC WMS Service (5) Citation (2) Contacts National Wildlife Health Center Ecosystems U.S. Geological Survey Rachel C Abbott Robin E Russell More... Categories Data (15) Data Release - Revised (1) Tag Types Place (14) Theme (15) Harvest Set (15) USGS Scientific Topic Keyword (9) Tag Schemes None (15) Common geographic areas (6) ISO 19115 Topic Category (12) USGS Thesaurus (3) none (1) View Results as: JSON ATOM CSV

SPV field trials bait uptake data These data were collected as part of a field trial to test the effectiveness of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available pairs at each location. Baits containing Rhodamine B, a biomarker, were distributed at each plot. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked, and sampled prairie dogs. Hair and whisker samples were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and the identity of all current-year and prior-year recaptures were recorded for each captured animal. In the laboratory, hair/whiskers were assessed for the presence... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Arizona, Awapa Plateau, Buffalo Gap National Grasslands, Cedar City, Charles M. Russell National Wildlife Refuge, All tags... Colorado, Coyote Basin, Espee Ranch, Gunnison, Larimer, Lower Brule, Montana, Pitchfork Ranch, Rita Blanca National Grasslands, South Dakota, Texas, USGS Science Data Catalog (SDC), Utah, Wind Cave National Park, biota, health, prairie dogs, sylvatic plague vaccine, Fewer tags In vitro expression, immunogenicity, and efficacy data from recombinant raccoon poxvirus-vectored rabies vaccine candidates tested in mice This work is part of an experimental trial to develop and assess novel recombinant raccoonpox virus (RCN) rabies vaccines in the mouse model, for potential use in bats. Briefly, our research group previously developed a recombinant RCN vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with rabies virus (RABV). We developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. In this data set, we share results of immunofluorescence assay... Categories: Data; Tags: A/J mice, National Wildlife Health Center, Madison, WI, RABV, USGS Science Data Catalog (SDC), University of Wisconsin-Madison, Madison, WI - Hanson Biomedical Sciences, All tags... Wildlife Disease, biota, rabies, rabies virus, raccoon poxvirus, recombinant, vaccine, Fewer tags Serology, survival, and detection of rabies data from vaccination trials in the common vampire bat (Desmodus rotundus) using a recombinant raccoon poxvirus-vectored mosaic rabies vaccine candidate This data set provides biological information from of a captive colony of common vampire bats (wild-caught and born in captivity) that were part of an experimental study on vaccination. It includes results obtained from different assays: RT-PCR, serum neutralization, Rabies direct-fluorescent antibody test, performed periodically during the study (Sep 2018 – May 2020). Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Rabies, San Luis Potosi, Mexico, Serology, USGS National Wildlife health Center, Madison, WI, USA, USGS Science Data Catalog (SDC), All tags... Wildlife Disease, biota, shedding, survival, vaccination, vaccine treatment, vampire bats, wildlife disease, Fewer tags Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed). Up on capture, we documented the trap numbers and the processed animals. We noted standard biological information (species, sex, age) and took samples (fleas, blood, hair and whiskers). When logistics allowed we also trapped diurnal animals. Hair and whisker samples were taped to a transparent sheet and scored for the presence (high dose 1, low dose 2) or absence (0) of Rhodamine B fluorescence (RB dataset) Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Awapa Plateau, Bend, Buffalo Gap National Grasslands and Wind Cave National Park, Charles M. Russell National Wildlife Refuge, Meeteetse East, All tags... Meeteetse West, Montana, Oral Sylvatic Plague Vaccine (SPV), Parowan area, Pitchfork Ranch, South Dakota, USGS Science Data Catalog (SDC), Utah, Vernal area, Wyoming, biota, health, Fewer tags Plague positive mouse fleas on mice prior to plague outbreaks in black-tailed and white-tailed prairie dogs In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed) and high elevation Utah plots where plague was active were more frequently trapped in 2014 and 2015. In the dataset the prevalence of flea infestation was recorded for the first annual summer sampling, it was summarized for all small rodent species caught and deer mice (Peromyscus maniculatus). Infestations were either for all flea species collected or for Aetheca wagneri only (our most abundant flea species). We used this data to assess if plague... Categories: Data; Tags: Montana, Northern grasshopper mice, South Dakota, USGS Science Data Catalog (SDC), Utah, All tags... Wildlife Disease, Wyoming, Yersinia pestis, biota, deer mice, fleas, mice, plague, Fewer tags Survival of prairie dogs (Cynomys spp.) challenged with Yersina pestis (plague) These data represent the number of days a prairie dog survived after challenge with highly virulent Yeresinia pestis. Categories: Data; Tags: Arizona, Colorado, South Dakota, Texas, USGS Science Data Catalog (SDC), All tags... Utah, Wildlife Disease, biota, disease susceptibility, plague, prairie dogs, survival analyses, Fewer tags Sylvatic plague vaccine field trials flea data These data were collected as part of a field trial to test the efficacy of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available sites at each location. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked and sampled prairie dogs for a minimum of 3 trap days. Both plots in a pair were trapped on the same day, and trap effort (number of traps and trap days) between plots of the same pair was similar with few exceptions. Fleas were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and foot length were recorded for each captured animal. In the laboratory,... Categories: Data, Data Release - Revised; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Awapa Plateau, Utah, USA, Buffalo Gap, South Dakota, USA, Cedar City, Utah, USA, Charles M Russell National Wildlife Refuge, Montana, USA, Coyote Basin, Utah, USA, All tags... Espee Ranch, Arizona, USA, Lower Brule, South Dakota, USA, Pitchfork Ranch, Wyoming, USA, Rita Blanca National Grasslands, Texas, USA, Sylvatic plague vaccine, USGS Science Data Catalog (SDC), Wind Cave National Park, South Dakota, USA, biota, Fewer tags Luminescence of AG129 mice infected with recombinant Monkeypox virus expressing firefly luciferase Adult AG129 mice (129/Sv background deficient in alpha/beta interferon (IFN-α/β) and IFN-Ɣ receptors) were infected with recombinant Monkeypox virus (MPXV)expressing firefly luciferase by either intranasal (IN) or intraperitoneal (IP) routes. Experimental infections were conducted in a BSL-3 laboratory at the USGS National Wildlife Health Center, with a clade II MPXV that expresses firefly luciferase, as described in Osorio et. al, 2009 (MPXV/USA/luc). Twelve AG129 mice (8 males and 4 females) were infected intranasally with 105 plaque forming units (PFU) (5 ul, each nostril) of MPXV/USA/luc. Three control mice were inoculated in the same manner with an equal volume of PBS. Bioluminescent imaging was performed using... Categories: Data; Tags: Experimental Infection Trial, In vivo imaging, Orthopoxvirus, USGS National Wildlife Health Center; Madison, WI, USGS Science Data Catalog (SDC), All tags... Wildlife Disease, biota, wildlife disease, zoonotic diseases, Fewer tags Monkeypox challenge of rope squirrels: data Monkeypox virus (MPXV) is a zoonotic disease endemic in Central and West Africa and is the most virulent orthopoxvirus affecting humans since the eradication of variola virus (VARV). In order to assess their reservoir potential, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication via in vivo bioluminescent imaging and viral shedding via culture and PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and... Categories: Data; Types: Citation, Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Congo (Democratic Republic of the), Monkeypox virus, USGS Science Data Catalog (SDC), biophotonic imaging, serology, All tags... viral shedding, Fewer tags Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and cleared the virus before the end of the study with no obvious clinical signs of disease. We additionally found no evidence of viral transmission to uninoculated subjects. These results indicate that while TABR are susceptible to SARS-CoV-2 infection, infection of wild populations of TABR would not likely cause mortality. Categories: Data; Tags: Mexican free-tailed bats, SARS-CoV-2, USGS National Wildlife Health Center Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, Fewer tags Data on Flea Parasitism and Annual Re-encounters of Utah Prairie Dogs at 5 colonies on the Awapa Plateau, Utah, USA, 2013-2016 Data on flea parasitism and annual re-encounters of Utah prairie dogs at 5 colonies on the Awapa Plateau, Utah, USA, June-August 2013-2016. Utah prairie dogs were live-trapped and sampled on 5 colonies along an elevation gradient from 2,645 m to 2,873 m. Upon first capture each year, we anesthetized each prairie dog and fleas on its body and combed the prairie dog as thoroughly as possible for 30 s to collect fleas. We recorded the age (juvenile/adult), sex, and mass (nearest 5 g) of each prairie dog and marked its ears and body with metal tags and passive integrated transponders, respectively, for permanent identification. We indexed each prairie dog's body condition as the ratio between its weight and hind-foot... Categories: Data; Tags: Cynomys, Flea, Plague, Prairie dog, USGS Science Data Catalog (SDC), All tags... Yersinia pestis, biota, Fewer tags Fleas collected from black-tailed prairie dog burrows in 2016 and 2017 The data are a list of the number and species of fleas that were collected from black-tailed prairie dog burrows in Montana, North Dakota, South Dakota, Colorado, and New Mexico. Fleas were collected by swabbing ~100 burrows at 3 sites at each of 6 sites. Burrows were sampled twice each summer during 2016 and 2017. Categories: Data; Tags: BLM Pecos District, Black-tailed prairie dogs, Buffalo Gap National Grassland, Charles M. Russell National Wildlife Refuge, Lower Brule Sioux Reservation, All tags... Oropsylla hirsuta, Oropsylla tuberculata, Polygenis gwyni, Pueblo Chemical Depot, Colorado, Pulex simulans, Theodore Roosevelt National Park South Unit, Thrassis fotus, UL Bend National Wildlife Refuge, USGS Science Data Catalog (SDC), Wildlife Disease, biota, burrows, fleas, Fewer tags Field efficacy trials with sylvatic plague vaccine These data were collected as part of a field trial to test the efficacy of a sylvatic plague vaccine. Treatment and control sites were selected randomly from the available sites at each location. Site pairs were a minimum of 20 acres, (with a few exceptions). Prairie dog trapping took place a minimum of two weeks post-baiting and trapping procedures were approved by the NWHC Animal Care and Use Committee as well as individual states as required. Categories: Data; Types: Citation; Tags: Arizona, Awapa Plateau, Buffalo Gap National Grasslands, Cedar City, Charles M. Russell National Wildlife Refuge, All tags... Colorado, Coyote Basin, Espee Ranch, Gunnison County, Larimer County, Lower Brule, Lower Brule, Montana, Pitchfork Ranch, Rita Blanca National Grasslands, South Dakota, Texas, USGS Science Data Catalog (SDC), Wind Cave, Wyoming, prairie dogs, Fewer tags Temperatures of black-tailed prairie dog burrows through the U.S. Great Plains This data is measurements of the temperature inside prairie dog burrows. The data were recorded every half hour during May, June, and August of 2016 and 2017 in New Mexico, Colorado, South Dakota, North Dakota, and Montana. Categories: Data; Tags: BLM Pecos District, NM, Black-tailed prairie dogs, Buffalo Gap National Grassland, SD, Charles M. Russell National Wildlife Refuge, MT, Lower Brule Sioux Reservation, SD, All tags... Pueblo Chemical Depot, CO, Theodore Roosevelt National Park South Unit, ND, UL Bend National Wildlife Refuge, MT, USGS Science Data Catalog (SDC), Wildlife Disease, burrows, fleas, microclimate, relative humidity, temperature, Fewer tags Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate This data set provides biological information from of a captive study of transgenic mice that received either passive serum transfers from black-footed ferrets (BFFs) that were vaccinated against SARS-CoV-2 or BFFs that were unvaccinated, or a monoclonal antibody against SARS-CoV-2 or lastly a placebo. After these treatments mice were challenged with SARS-CoV-2. The data included here have viral loads in the lungs of treated mice, as well as histological results from various tissues collected at the end of the study. Categories: Data; Tags: Passive serum transfer, SARS-CoV-2, USGS National Wildlife Health Center, Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, black-footed ferrets, experimental challenge, immunogenicity and susceptibility, mustelids, pathology, vaccination, viral loads, Fewer tags